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Projects/Programs

Keyword

Organizational Unit

We have developed 3D orientation imaging methods for both infrared (IR) and coherent anti-Stokes Raman scattering (CARS) signals. The two approaches are complementary to each other. The IR-based 3D orientation imaging is broadly applicable to any pair of non-parallel IR transition dipole moments

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Pathogen Detection and Identification: The Next Generation of Analyses

Ongoing

Human disease resulting from pathogens represents a tremendous burden on the health care system. Many hospitals have adopted new, rapid polymerase chain reaction (PCR)-based detection systems because the time to diagnose and begin effective treatment has a direct impact on patient survival and

Photo montage showing fill the airplane with fire-fighting foam, a spilled glass of wine on the tablecloth. Cleaning clothes and furniture from stains, Discarded Fast Food Wrappers Littered on Ground, wateproof jacket with water droplets on it. Jacket using the gore-tex technology.

Per- and Polyfluoroalkyl Substances (PFAS)

Ongoing

Perfluorinated and polyfluorinated alkyl substances (PFAS) constitute a family of compounds that are distinguished by partial or complete fluorination of alkyl hydrocarbons. Dubbed “forever chemicals”, the unique chemistry of PFAS imparts a high level of stability, making the compounds resistant to

Performance Evaluation of Automatic Exposure Notification with Bluetooth-based Proximity Detection

Ongoing

The proximity detection mechanism in current automatic exposure notification systems is typically based on the Bluetooth signal strength from the individual’s mobile phone. However, there is an underlying error in this proximity detection methodology that could result in wrong exposure decisions i.e

Performance Metrics for Proteomics

Ongoing

Intended Impact Proteomics experiments designed to identify protein markers specific to a particular disease or disease state rely on repeatable identification of proteins within a control set. Repeatability provides statistical assurance that clear differences between two sets (e.g. sick vs

Photonic Dosimetry

Ongoing

With this effort, NIST is responding to industry needs for traceable, measurement solutions that can resolve spatial variations of absorbed dose at the level of individual components on a silicon wafer or bacteria on surgical instruments. Presently, there is only limited traceability to national

Photopolymerization for Biomaterials

Completed

This project responds to priorities of National Institute of Dental and Craniofacial Research (NIDCR/NIH) for oral health by establishing clinical guidance/standards for adequate photocuring of restorative dental composites. Also, this project is intended to advance the knowledge relevant to thermal

Point-of-Care Pharmaceutical Manufacturing & Precision Medicine

Ongoing

We are employing quality by design (QbD) and risk assessment (cause-and-effect) approaches to investigate production technologies ( e.g., drop on demand or inkjet printing platforms) and appropriate analytical control strategies for point-of-care manufacture of narrow therapeutic index drugs

Protein Preservation

Completed

This project is an integral part of a cooperative effort – led by NIST, and partially funded by NIH/ NIBIB – which includes the University of Wisconsin, the University of Colorado, and the University of Connecticut. We use the following approach to develop measurement solutions for biopharmaceutical

Quantitative Flow Cytometry Measurements

Ongoing

Flow cytometry is an essential tool for: basic biotechnological and immunological research, the clinical discovery of potential therapeutics, development and approval of drugs and devices, disease diagnosis, and therapeutic treatment and monitoring For example, flow cytometry is commonly used in pre

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Radio Frequency Propagation Characterization, Statistical Channel Modeling, Interference analysis and Mitigation in Body Area Networks

Ongoing

A body area network (BAN) is a wireless network consisting of small, low power, wearable, implantable or ingestible devices. Integrated with various biomedical sensors or actuators, these devices basically provide data communication paths between the human body and the external world. As such, they

Reliable Vaccine Storage

Completed

Our group examines best practices for the storage and monitoring of vaccines in partnership with the Centers for Disease Control and Prevention’s (CDC) Vaccine Supply and Assurance Branch. Vaccines are highly temperature sensitive, and inappropriate storage conditions can render them ineffective

RMAT Laboratory Programs

Ongoing

Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not

Serology and Neutralization Assays for COVID-19

Ongoing

Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have

Single-Cell Manipulation and Measurement

Ongoing

Mammalian cells are an important source material for applications in regenerative medicine, gene therapy, engineering biology, and genome editing. Traditional tools for manipulating mammalian cells operate at a bulk cell population level without precision manipulation of individual cells

DNA Plasmid containing 13 PCR targets for water quality testing

Standards for Assessing the Safety of Recreational Waters from Fecal Contamination

Completed

Over 286 million Americans get their tap water from a community water system. To assure the water safety, bacterial testing must be performed regularly. Major sources of contaminants include human derived sources, such as sewage leaks or combined sewer overflows; and agricultural sources, such as

Standards Development to Ensure Reliable Breath Analysis in the Field

Ongoing

Numerous volatile organic compounds (VOCs) have been identified in human breath. These compounds can be produced by the body or by organisms in the body (e.g., bacteria or viruses) and provide a non-invasive window into human health. Inexpensive point-of-care devices are being developed to diagnose

Standards for Metagenomics

Ongoing

Metagenomics is a powerful technology combining high-throughput (a.k.a. “next-generation”) sequencing with advanced bioinformatics to identify and quantify mixtures of organisms in a sample. Metagenomics workflows consist of 4 main steps: Sample collection: location, amount, storage, etc. Extraction

Standards to Support Biological Detection in the Field

Ongoing

NIST is working in partnership with the Department of Homeland Security Science and Technology Directorate (DHS S&T) to develop methods, guidance, standards, and reference materials to support and improve our Nation's ability to detect and respond to suspected biothreat incidents. Specific

First responders collecting samples of the yeast surrogate material during a field exercise. (Credit: NIST)

Standards to Support Biothreat Detection

Ongoing

Since 9/11 and the subsequent anthrax attacks in 2001, suspicious white powder incidents continue to occur within the US, often requiring Federal law enforcement response. Suspect samples are sent to public health laboratories for confirmatory evaluation, which can take hours to weeks to receive

Storage Stability of Freeze-Dried Proteins

Completed

Intended Impact Work in this project will enable industry to develop rational formulation approaches for freeze-drying, thus facilitating the manufacture safer, longer lasting biopharmaceuticals, with less time and resource cost required for stabilization. Biopharmaceuticals are the fastest growing

Structural Databases

Completed

Some of the current projects are a) http://xpdb.nist.gov/hivsdb/hivsdb.html HIVSDB b) http://bioinfo.nist.gov/SemanticWeb_pr2d/chemblast.do HIVSDB Chem-BLAST c) http://bioinfo.nist.gov/biofuels/ A resource for Biofuels d) http://xpdb.nist.gov/pdb/chemblast.html A ligand gateway for the Protein Data

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

Ongoing

Nationwide, first responders regularly encounter suspicious materials that might be biological threat agents. Routine training and process evaluations help ensure workflows, operators, and technologies are working together properly within the concept of operations. Biological test materials have the

Health

Projects/Programs

3D Orientation Imaging

Orthogonal Measurements

Pathogen Detection and Identification: The Next Generation of Analyses

Per- and Polyfluoroalkyl Substances (PFAS)

Performance Evaluation of Automatic Exposure Notification with Bluetooth-based Proximity Detection

Performance Metrics for Proteomics

Photonic Dosimetry

Photopolymerization for Biomaterials

Point-of-Care Pharmaceutical Manufacturing & Precision Medicine

Protein Preservation

Quantitative Flow Cytometry Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Radio Frequency Propagation Characterization, Statistical Channel Modeling, Interference analysis and Mitigation in Body Area Networks

Reliable Vaccine Storage

RMAT Laboratory Programs

Serology and Neutralization Assays for COVID-19

Single-Cell Manipulation and Measurement

Standards for Assessing the Safety of Recreational Waters from Fecal Contamination

Standards Development to Ensure Reliable Breath Analysis in the Field

Standards for Metagenomics

Standards to Support Biological Detection in the Field

Standards to Support Biothreat Detection

Storage Stability of Freeze-Dried Proteins

Structural Databases

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

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