Projects/Programs

Displaying 1 - 25 of 136

AAPS Interlaboratory Study: Protein Aggregation and Immunogenicity

Ongoing

To ensure the safety and efficacy of protein-based biotherapeutics, manufacturers strive to avoid unwanted immune responses caused by the biotherapeutic. Protein immunogenicity is a complex topic and depends on multiple factors related to the product, patient profile, and treatment. 1 , 2

Advanced Spectral Analysis: Two-Dimensional Correlation Spectroscopy (2DCOS)

Ongoing

When a series of spectra are acquired from a sample as a function of temperature, pressure, electric field strength, concentration, or any experimental parameters, conventional spectral analysis is performed by monitoring the intensity change at a specific frequency. However, such a single frequency

Assessing the Environmental, Health and Safety Impact of Nanoparticles

Completed

Impact Nanomaterials have already been incorporated into a large range of commercial products including pharmaceuticals, sunscreens, automobile additives, personal care products, detergents and plastics. Although nanotechnology is changing the way we live, the accidental release of nanomaterials

Biofabrication of Tissue Engineered Constructs

Ongoing

OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series

Biofilm Models to Evaluate Structure-Function Relationships

Ongoing

Biofilms are complex 3D microbial structures that exist throughout nature including on our skin and gut mucosa, in waterways, and in soil. They are typically composed of multiple microbial species surrounded by proteins, polysaccharides, and extracellular DNA woven together into a hydrated matrix

Bioimaging

Completed

Imaging and image analysis are used ubiquitously in biological and biomedical research. One of the great advantages of imaging is its intuitive information content; much can be learned from just a glance at the data. On the other hand, these data generally contain a very large amount of untapped

Biomanufacturing Initiative

Ongoing

The objective of the NIST biomanufacturing program is to support US biopharmaceutical industry delivery of high quality and low cost protein drugs around the world. The mission is fulfilled through the development of standards, measurement science, and state of the art tools that support advances in

Biomaterials for Oral Health

Completed

NIST along with its partners has a long commitment to scientific excellence with demonstrated impact in improving the oral health of the public. NIST researchers are leaders in this field and create a community by engaging other researchers, industry, and regulatory agencies to address urgent needs

Bioprocessing Laboratory

Ongoing

Current Capabilities Computer controlled liquid nitrogen biostorage system Two culture areas including 4 monitored CO2 incubators 2 Six-foot BSLII biosafety cabinets Measurements- cell count and concentration, STR typing, flow cytometry, cell volume size distribution, conventional transmission and

Biosurveillance and Pathogen Detection

Ongoing

NIST has established partnerships with other federal agencies to develop standards and measurement solutions to support biosurveillance. These efforts are supported in part through interagency agreements with partner agencies listed below to address their standards needs. PROGRAMS Standards for

Broadband Coherent Anti-Stokes Raman Scattering (BCARS) Microscopy

Ongoing

Impact There is a need for label-free chemical microscopy in medicine, biology and materials science. Most of the current methods use chemical labels that often disturb the distribution and nature of chemical components being investigated. The method we are developing enables noninvasive and rapid

Building Measurement Confidence for Cell Characterization

Ongoing

NIST collaborates with key stakeholders in industry, other government agencies, and academia to develop measurement assurance strategies and advanced measurement capabilities for cell characterization. Data and concepts developed through workshops and laboratory programs inform the development of

Calibration Services: High-Dose Dosimetry

Ongoing

NIST developed the alanine dosimetry system in the early 1990's to replace radiochromic dye film dosimeters. Later in the decade the alanine system was firmly established as a transfer service for high-dose radiation dosimetry and an integral part of the internal calibration scheme supporting these

Cancer Biomarker Measurements and Collaborations

Ongoing

Collaborations and Cooperative Research Agreements We welcome collaborations and can help with assay validation and development of reference materials. Below are some examples of our collaborations and ways we can develop test materials. Early Detection Research Network (EDRN): Interagency agreement

Cancer Genome in a Bottle

Ongoing

Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cell Counting for Cell Therapies

Ongoing

Recent Publications and Resources for Cell Counting and Related Measurements Tools NOW AVAILABLE : COMET (Counting Method Evaluation Tool) , A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391-2:2019 https://cell-counting.shinyapps.io/COMET/ Standards ISO

Cell Line Authentication

Ongoing

Cell Line Authentication – The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed multiplex polymerase chain reaction (PCR

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Cell-based Assay for Mechanistically-based Prediction of Cytoxicity

Completed

Ricin is a type 2 ribosome inactivating protein (RIP) belonging to the A-B family of toxins and is of particular interest because of its potential use as a biological weapon and the dearth of rapid and sensitive assays that could assist detection and decontamination. The ricin toxin inhibits protein

Characterization of Dolphin Lung Endothelial Cells Through Quantitative Imaging

Completed

Due to their characteristics (long life span, feed at a high trophic level, extensive fat stores that can act as a deposit for anthropogenic toxin) dolphins have been proposed as a good sentinel organism in aquatic and coastal environments to monitor and understand the impact of infectious diseases

Chemical Foundations for a Cannabis Breathalyzer

Ongoing

NIST's initial work in cannabis science was a series of measurements of the vapor pressure of two important cannabinoids: delta-9-tetrahydrocannabinol (THC) and cannabidiol (CBD). Our current effort is funded in part by interagency agreements with the National Institute of Justice and by the NIST

Classification Methods for the NIST Biorepository

Ongoing

NIST has been archiving biological and environmental specimens, including marine animal and environmental samples, for over 40 years. Currently, these biospecimens are stored at cryogenic temperatures in the NIST Biorepository located at the Hollings Marine Laboratory in Charleston, SC. Various

Clinical and Forensic Reference Materials

Ongoing

Below is a listing of the reference materials currently produced by the Applied Genetics group. Clinical Diagnostics SRM 2393 - CAG Repeat Length Mutation in Huntington`s Disease SRM 2365 - BK Virus DNA Quantitative Standard SRM 2366a - Cytomegalovirus DNA (Towne Δ147 BAC) for DNA SRM 2367 - JC

Combating Antibiotic Resistance

Ongoing

We are developing in vitro systems to generate controlled chemical and community environments that mimic the features of real-world that are important determinants for evolutionary rate and resistance potential. In addition, we are developing tools to isolate and analyze single bacteria along the

Comparative Mammalian Proteome Aggregator Resource (CoMPARe) Program

Ongoing

Comparative proteomics strives to gain insight into key underlying molecular changes that result in unique phenotypes across related taxa. Proteomic analysis complements comparative genomics by providing evidence of protein abundance, orthogonal to gene copy number and amount of transcript. To

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