Projects/Programs

Displaying 1 - 25 of 66

Assessing the Environmental, Health and Safety Impact of Nanoparticles

Completed

Impact Nanomaterials have already been incorporated into a large range of commercial products including pharmaceuticals, sunscreens, automobile additives, personal care products, detergents and plastics. Although nanotechnology is changing the way we live, the accidental release of nanomaterials

Automated Live Cell Imaging for Quantification of Catalytic Rates in Individual Cells

Completed

In this study, the rates at which large numbers of individual living cells enzymatically degrade green fluorescent protein (GFP) was examined using automated fluorescence microscopy. A clonal population of NIH3T3 fibroblasts that resulted from stable transfection with a construct containing the

$Autonomous materials metrology diffraction measurement performed at SLAC$

Autonomous Systems for Materials Research and Metrology: Accelerating Discovery and Democratizing Science

Ongoing

Materials scientists often need to search high dimensional spaces for knowledge. For example, a scientist may be interested in learning how a material property (e.g. hardness) changes over many (for example, 10) values of a synthesis or processing parameter (e.g., annealing temperature). Such an

Biofabrication of Tissue Engineered Constructs

Ongoing

OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series

Bioimaging

Completed

Imaging and image analysis are used ubiquitously in biological and biomedical research. One of the great advantages of imaging is its intuitive information content; much can be learned from just a glance at the data. On the other hand, these data generally contain a very large amount of untapped

Bioprocessing Laboratory

Ongoing

Current Capabilities Computer controlled liquid nitrogen biostorage system Two culture areas including 4 monitored CO2 incubators 2 Six-foot BSLII biosafety cabinets Measurements- cell count and concentration, STR typing, flow cytometry, cell volume size distribution, conventional transmission and

Building Measurement Confidence for Cell Characterization

Ongoing

NIST collaborates with key stakeholders in industry, other government agencies, and academia to develop measurement assurance strategies and advanced measurement capabilities for cell characterization. Data and concepts developed through workshops and laboratory programs inform the development of

Cancer Genome in a Bottle

Ongoing

Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cell Counting for Cell Therapies

Ongoing

Recent Publications and Resources for Cell Counting and Related Measurements Tools NOW AVAILABLE : COMET (Counting Method Evaluation Tool) , A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391-2:2019 https://cell-counting.shinyapps.io/COMET/ Standards ISO

Cell Viability for Advanced Therapies

Ongoing

In the Biosystems and Biomaterials Division at NIST, we have established several technical programs to support the development and implementation of cell viability assays in advanced therapies. These programs address key needs in cell viability assay development including selection and development

Cell-based Assay for Mechanistically-based Prediction of Cytoxicity

Completed

Ricin is a type 2 ribosome inactivating protein (RIP) belonging to the A-B family of toxins and is of particular interest because of its potential use as a biological weapon and the dearth of rapid and sensitive assays that could assist detection and decontamination. The ricin toxin inhibits protein

Characterization of Dolphin Lung Endothelial Cells Through Quantitative Imaging

Completed

Due to their characteristics (long life span, feed at a high trophic level, extensive fat stores that can act as a deposit for anthropogenic toxin) dolphins have been proposed as a good sentinel organism in aquatic and coastal environments to monitor and understand the impact of infectious diseases

Chinese hamster ovary (CHO) cell line authentication

Ongoing

Intended impact To develop a PCR based multiplex assay that can be used to authenticate Chinese hamster ovary cell lines which are important in the production of biological therapeutics. Objectives Identify tetranucleotide STR markers by searching the CHO genome using BLAST Design and optimize a

Clinical and Forensic Reference Materials

Ongoing

Below is a listing of the reference materials currently produced by the Applied Genetics group. Clinical Diagnostics SRM 2393 - CAG Repeat Length Mutation in Huntington`s Disease SRM 2365 - BK Virus DNA Quantitative Standard SRM 2366a - Cytomegalovirus DNA (Towne Δ147 BAC) for DNA SRM 2367 - JC

Combating Antibiotic Resistance

Ongoing

We are developing in vitro systems to generate controlled chemical and community environments that mimic the features of real-world that are important determinants for evolutionary rate and resistance potential. In addition, we are developing tools to isolate and analyze single bacteria along the

Comparative Mammalian Proteome Aggregator Resource (CoMPARe) Program

Ongoing

Comparative proteomics strives to gain insight into key underlying molecular changes that result in unique phenotypes across related taxa. Proteomic analysis complements comparative genomics by providing evidence of protein abundance, orthogonal to gene copy number and amount of transcript. To

Comparative Serum Proteomics Project

Ongoing

State-of-the-art biomolecular analysis is no longer limited to model organisms and is becoming routine in non-model organisms. Major drivers of this emerging bioanalytical capacity include increasing accessibility and quality of sequenced genomes as well as high-resolution fast-duty cycle mass

Comparing the Biological Responses of Photoluminescent Silicon Nanoparticles with Silicon Micron-sized Particles

Completed

Intended Impact Technology is now available to produce nanoparticles of uniform size and shape which may allow their use in a wide range of applications from drug delivery to water purification. The high surface area to volume ratio of nanoparticles makes them particularly good catalysts as well as

Controlling Microfluidic Cell Cultures

Completed

Intended impact: Cell behavior is powerfully modulated by local extracellular cues. However, the number of parameters that affect cell behavior is large, and include soluble signaling molecules, dissolved gases, the chemistry and mechanics of the extracellular matrix, and the proximity and behavior

DARPA Friend or Foe (FoF) – Independent Verification and Validation (IV&V)

Completed

Vision Validate new measurement platforms for identifying commensal (friend) and pathogenic (foe) microorganisms Goal Provide well-characterized, microbial community reference materials of increasing complexity Approach Start with well-characterized pure cultures and scale up to complex consortia in

Development of Biomarker-based Systems for Cancer Detection and Therapy

Completed

Intended impact Although significant progress has been made in both the understanding and treatment of cancer during the last thirty years, it remains the second leading cause of death in the US. The cancer community has set a goal to eliminate cancer-related suffering and death by 2015. To achieve

Development of Enhanced mRNA Amplification Using Microfluidics

Completed

Intended Impact Tissue samples frequently contain multiple cell types. New methods (e.g. laser capture microdissection) enable the segregation of small quantities of individual cells and cell types, but the current methods to analyze global gene expression do not provide highly efficient profiling

Development of the NIST Prototype Advanced Biometrology Laboratory

Ongoing

Currently, NIST is investing in a new type of metrology laboratory that is focused on the unique reference material and high quality assay protocols that are needed in the regenerative medicine and biotechnology communities. The laboratory design is centered on automation that can set up 1000’s of

Ecogenotoxicity Measurements of Nanoparticles

Completed

Intended Impact Understanding the potential environmental fate of NPs has direct implications to the US economy, as potential environmental and human health impacts of NPs is one of the main factors slowing the commercialization of this technology. Reducing the uncertainty regarding the

Engineering of G Protein-Coupled Receptors

Ongoing

A trait of all life is the ability to sense and respond to changing environments, and the largest family of eukaryotic proteins that sense and respond to extracellular signals are called G Protein-Coupled Receptors (GPCRs). In humans, over 800 GPCRs detect a wide range of biological and chemical

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