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Bioscience

Projects/Programs

Keyword

Organizational Unit

Resonating Platforms for Microbial, Environmental, and Materials Sensing

Ongoing

The high sensitivity of resonating piezoelectric crystals to surface perturbations has led to quartz crystal microbalances (QCMs) being widely used to measure changes in mass of thin films and adhered nanoparticles or cells. This project proceeds beyond this traditional sensing approach with quartz

RMAT Laboratory Programs

Ongoing

Examples of Current Activities: Cell Counting and viability are key measurements critical for decision making from R&D to manufacturing. We recently developed an approach for evaluating the quality of cell counting measurements through experimental design and statistical analysis that does not

RNA Computation and Metrology for Engineering Biology

Ongoing

RNA computation RNA computation, i.e., programmed interactions between RNA molecules that process information, is an increasingly useful synthetic biology tool with applications spanning in vitro biosensing, synthetic cells, and cellular control. We have developed cotranscriptionally encoded RNA

Epidemiologist in protective suit, mask and glasses works with patient swabs to detect specific region of 2019-nCoV virus causing Covid-19

SARS-CoV-2 Research Grade Test Material

Completed

In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test

Serology and Neutralization Assays for COVID-19

Ongoing

Multiplexed bead-based SARS-CoV-2 serological assay We have developed a validated multiplexed bead-based SARS-CoV-2 serological assay that measures different antibodies produced by a patient (IgG, IgM, IgA). In collaboration with the NIST Applied and Computational Mathematics Division, we have

Single-Cell Manipulation and Measurement

Ongoing

Mammalian cells are an important source material for applications in regenerative medicine, gene therapy, engineering biology, and genome editing. Traditional tools for manipulating mammalian cells operate at a bulk cell population level without precision manipulation of individual cells

Single-molecule biosensors

Ongoing

We are developing new tools to investigate the energy landscape of single-molecule sensors. Our goal is to apply a wide-ranging array of technologies to probe the critical physicochemical properties of nanopore biosensors. These properties include the free energy of confinement for polymers as they

SMART Contrast Agents

Ongoing

Work on smart agents is focused on developing new micro- and nanoparticle- based contrast agents for MRI and new MR imaging and sensing schemes. These include synthetic antiferromagnet nanoparticles as potential new contrast agents, high-moment iron microparticles for enhanced T2* contrast for in

DNA Plasmid containing 13 PCR targets for water quality testing

Standards for Assessing the Safety of Recreational Waters from Fecal Contamination

Completed

Over 286 million Americans get their tap water from a community water system. To assure the water safety, bacterial testing must be performed regularly. Major sources of contaminants include human derived sources, such as sewage leaks or combined sewer overflows; and agricultural sources, such as

Standards Development to Ensure Reliable Breath Analysis in the Field

Ongoing

Numerous volatile organic compounds (VOCs) have been identified in human breath. These compounds can be produced by the body or by organisms in the body (e.g., bacteria or viruses) and provide a non-invasive window into human health. Inexpensive point-of-care devices are being developed to diagnose

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

Completed

Intended Impact The lack of quantitative imaging methods and reagents impedes the validation of image-based biomarkers. Improved methods for biomarker quantification and an established traceable value assignment would be useful to customers in a wide range of areas that include clinical diagnostics

Standards for Metagenomics

Ongoing

Metagenomics is a powerful technology combining high-throughput (a.k.a. “next-generation”) sequencing with advanced bioinformatics to identify and quantify mixtures of organisms in a sample. Metagenomics workflows consist of 4 main steps: Sample collection: location, amount, storage, etc. Extraction

Standards to Support Biological Detection in the Field

Ongoing

NIST is working in partnership with the Department of Homeland Security Science and Technology Directorate (DHS S&T) to develop methods, guidance, standards, and reference materials to support and improve our Nation's ability to detect and respond to suspected biothreat incidents. Specific

First responders collecting samples of the yeast surrogate material during a field exercise. (Credit: NIST)

Standards to Support Biothreat Detection

Ongoing

Since 9/11 and the subsequent anthrax attacks in 2001, suspicious white powder incidents continue to occur within the US, often requiring Federal law enforcement response. Suspect samples are sent to public health laboratories for confirmatory evaluation, which can take hours to weeks to receive

This figure shows a microscopic image of a field of cells, some of which are pseudocolored red to indicate that these cells remain pluripotent.

Stem Cell Metrology: WSDOM

Ongoing

Stem cells are critical starting materials for advanced therapies and diagnostic devices. The most commonly used are induced pluripotent stem cells (iPSCs) which can be created with any individual’s skin cells, and then can be coaxed to become cells for any tissue of the body. Therapies and

Steroid Hormone Suite Analysis

Ongoing

Steroid hormones are signaling molecules that are major regulators of development, reproduction, stress, and growth. Steroids are synthesized from cholesterol through a series of enzymes, resulting in hormones with distinct biological activities. Although hormones are similar in structure, and

Storage Stability of Freeze-Dried Proteins

Completed

Intended Impact Work in this project will enable industry to develop rational formulation approaches for freeze-drying, thus facilitating the manufacture safer, longer lasting biopharmaceuticals, with less time and resource cost required for stabilization. Biopharmaceuticals are the fastest growing

Structural Databases

Completed

Some of the current projects are a) http://xpdb.nist.gov/hivsdb/hivsdb.html HIVSDB b) http://bioinfo.nist.gov/SemanticWeb_pr2d/chemblast.do HIVSDB Chem-BLAST c) http://bioinfo.nist.gov/biofuels/ A resource for Biofuels d) http://xpdb.nist.gov/pdb/chemblast.html A ligand gateway for the Protein Data

Surrogate Materials to Support Training in Biological Sampling and Detection in the Field

Ongoing

Nationwide, first responders regularly encounter suspicious materials that might be biological threat agents. Routine training and process evaluations help ensure workflows, operators, and technologies are working together properly within the concept of operations. Biological test materials have the

Surrogate protein particle standards

Ongoing

Intended Impact This work will enable industry to obtain accurate, reproducible counts of protein particles, and to differentiate protein particles from other particle types, resulting in greater drug efficacy and safety. Objectives Create a polydisperse suspension of polymer particles that mimic

Synthetic Biology

Ongoing

New measurement methods and tools are required for biology to emerge fully as an enabling, practical platform for engineering. The Cellular Engineering Group works to provide a foundation of measurement assurance to support the control and rational design of biological function. Through state of the

Research Grade Testing Material (RGTM 10212), Synthetic Fecal Metabolite Calibrant (Credit: NIST)

Synthetic Fecal Metabolite Calibrant Solution (RGTM 10212)

Ongoing

Metabolomics is an exciting and dynamic field in life sciences that harnesses cutting-edge technologies to identify and characterize small molecules, or metabolites, within cells, tissues, or organisms, collectively known as the metabolome. The wealth of evidence in the literature underscores the

U.S. TAG for ISO/TC276: Biotechnology

Ongoing

Get involved: Interested U.S. Stakeholders may participate in the US TAG at no cost. Membership provides access to all ISO/TC 276 and US TAG documents, including standards under development. Please click here to join. Contribute to standards development: Please click here to review, submit comment

Tandem Mass Spectral Library

Ongoing

Intended Impact Modern mass spectrometers commonly generate ions by electrospray mass spectrometry which are separated and then fragmented by collisional dissociation. This process yields fragment ion ‘fingerprints’ (or mass spectra) that are widely used to identify the individual components in a

Targeting Membrane Proteins

Ongoing

Membrane proteins constitute approximately 30% of all cellular proteins. Nevertheless, this group of proteins is traditionally understudied due to limitations of the available analytical tools. Given the importance of membrane proteins in the various cellular processes and their potential as drug

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