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Bioscience

Projects/Programs

Keyword

Organizational Unit

Multiparametric Measurement and Analysis of Cell Behavior on Extracellular Matrices

Completed

The rational design of biomaterials which can elicit desired cellular responses is an important requirement for tissue engineering. Understanding the role of different cues that extracellular matrices provide cells is an important component of achieving this objective. Type 1 collagen is a

Nanotube Metrology

Completed

Single-wall carbon nanotubes (SWCNTs) are a tubular form of carbon consisting of a single shell of sp2 bonded carbon with a nanometer scale diameter that have many predicted properties superior to other available materials. However, every production technique for SWCNTs produces many different

Next Generation Metrics for Cell-Based Therapies and Regenerative Medicines

Ongoing

The science of human health and disease often includes studies of cells, the smallest unit of biological complexity. Microscopic imaging is an important tool in these studies and can provide morphological and molecular information. Molecular microscopic data is most often collected in a static mode

The NIST Biorepository

Ongoing

The NBSB was established in 1979 at NIST in Gaithersburg, MD with a pilot project archiving human liver samples associated with collections through the Environmental Protection Agency (EPA). New collaborations and banking projects were established including the National Oceanic and Atmospheric

NIST/EDRN Cancer Biomarker Collaboration

Completed

Our lab is one of the EDRN Biomarker Reference Laboratories working with the EDRN discovery labs to improve the quality of the measurements done on new cancer biomarkers for early detection of cancer and for cancer risk. TECHNICAL APPROACH Evaluate current assays used for detection of cancer

NIST/FDA CVM Evaluation of Assays and Control Materials for Characterizing Genome-Edited Animal Biotechnology Products

Ongoing

FDA CVM oversees regulation of IGAs in animals, including those that have been created using genome editing technologies. Characterization of these IGAs is an important part of the regulatory process to ensure that the edit to the animal’s genome is what it is intended to be and to identify any

NIST Flow Cytometry Standards Consortium 

Ongoing

Advances in cell and gene-based therapeutics as well as other regenerative medicine products have increased the need for high quality, robust, and validated measurements for cell characterization. Flow cytometry, including imaging cytometry, has emerged as an important platform due to its ability to

NIST Genome Editing Consortium: Working Group 2 - Data & Metadata

Ongoing

Develop a metadata schema for genome editing experiments Develop a way of curating and entry that is low burden for user Release control data with linked metadata PROGRESS Phase1 metadata entries and templates completed In process of compiling and testing use cases, user interfaces, and

NIST Genome Editing Consortium

Ongoing

Targeted genome editing , a method used to alter the DNA of living cells at desired locations, is poised to revolutionize science and medicine. To fight diseases, novel genome edited therapeutics, including those for use in regenerative medicine and infectious diseases, are being developed. Many

NIST Genome Editing Consortium: Working Group 1 - Specificity Measurements

Ongoing

Genome editing technologies are able to produce DNA sequence changes of a variety of sizes and at a variety of frequencies in a population of edited cells. Having increased confidence in reliably detecting the sequences generated by a genome editing process was a need area identified. Project 1

NIST Genome Editing Lexicon

Ongoing

The definitions are worded with the intention that additional context may be added with supplementary language when they are used. It is also recognized that genome editing is a rapidly evolving biotechnology and additional terms and definitions will be needed as genome editing technologies mature

NIST Genome Editing Program

Ongoing

For genome editing systems to reach their full potential in research and commercial products, new measurement tools, capabilities, and standards must be developed to efficiently implement and assess the performance of these editing technologies, as well as to evaluate the utility of resulting

NIST HER2 Genomic DNA Standard Reference Material, SRM® 2373

Ongoing

The amplification of the gene for HER2 occurs in approximately 20 to 25% of breast cancers. The accurate measurement of this biomarker is important for the proper treatment with anti-HER2 therapeutics. Clinical laboratories are beginning to utilize accurate and sensitive diagnostic tests based on

NIST Living Measurement Systems Foundry

Ongoing

To enable the production of high-throughput, high-quality data that meets the rapidly evolving needs of current and future stakeholders, NIST has established an automation facility for the growth, manipulation, sample preparation, and measurement of engineered microbes. The core of the facility

NIST Microbial Strain Collection

Ongoing

Microorganisms of interest include but are not limited to those relevant to: biomanufacturing of advanced therapy products; rapid microbial testing; microbial therapeutics (e.g., live biotherapeutic products, probiotics, and fecal microbiota transplantations (FMTs)); infectious disease

NISTmAb RM8671 vial and 3D printed model

NIST Monoclonal Antibody Reference Material 8671

Ongoing

The NISTmAb material is a recombinant humanized IgG1κ expressed in murine suspension culture. It is an »150 kDa homodimer of two identical light chains and two identical heavy chains linked through both inter- and intra-chain disulfide bonds. The protein has low abundance post-translational

NIST/NIBIB Medical Imaging Phantom Lending Library

Ongoing

Phantoms are calibration objects that can be used to assess the accuracy, stability, and comparability of medical imaging systems. The NIST/ISMRM MRI system phantom can be used to assess scanner geometric distortion, image uniformity, signal to noise ratio (SNR), resolution, slice profile and

NIST Rapid Microbial Testing Methods Consortium

Ongoing

The safety and quality of advanced therapies, including cell therapy, gene therapy, and tissue engineered products, must be maintained prior to patient administration. The culture-based compendial methods currently used to assess product purity (specifically to ensure absence of microbial

NIST Urine Albumin Standardization Program

Ongoing

Kidney disease is a major global economic and public health issue, with chronic kidney disease (CKD) representing one of the top 10 most prominent causes of death worldwide. The public health and economic impact of CKD and other kidney diseases has led to the need for the accurate detection of

Test tube illustrating NIST cell-free workshop topics

NIST Workshops on Cell-Free Expression Systems

Completed

NIST has co-hosted two cell-free workshops. NIST CELL-FREE (Comparable Engineered Living Lysates For Research Education and Entrepreneurship) Workshop (2019) The NIST CELL-FREE workshop took place in La Jolla, CA in February 2019, in collaboration with the J. Craig Venter Institute (JCVI). The

NIST23: Updates to the NIST Tandem and Electron Ionization Spectral Libraries

Ongoing

NIST Tandem Mass Spectral Library, 2023 release 51,501compounds 60% increase over the 2020 release 399,267 precursor Ions 2.4 million spectra Fragmentation methods 49,590 HRAM (high res accurate mass) compounds 51,292 QTOF, HCD, IT-HRAM, QqQ compounds 49,561 ion trap compounds (low res., up to MS 4)

NISTCHO

Ongoing

NISTCHO, a new mammalian cell line of interest to the biopharmaceutical and biomanufacturing communities, is now available from the National Institute of Standards and Technology. This Chinese hamster ovary (CHO) cell line has been engineered by MilliporeSigma to express a non-originator version of

Omics Technological Advances in Aquaculture

Ongoing

According to the NOAA Fisheries of the U.S. report, in 2020 U.S. seafood consumption was estimated at ~6B pounds (~2.7 x 109 kg). Of that, 85% is imported and U.S. exports have seen a significant decline. Globally, the U.S. ranked only 18th in aquaculture production (2020). NIST aims to enhance the

Ontologies and Interoperability in Evolutionary Comparative Analysis

Completed

Intended impact Nearly all scientists who regularly use online resources on genes, proteins and genomes make use of comparative data to advance biomedical research. For instance, researchers often make useful inferences by comparing human genes (as well as proteins, reactions, interactions, pathways

Optical/electrical particle measurement system

Ongoing

Intended Impact The results of this work should provide instrument manufacturers with data, methods, and algorithms for enhancing the clarity and accuracy of information their instruments provide to their customers. Users of these instruments (pharma companies) and regulators, will have better

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