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Search Publications by: Katherine Butler Gettings (Fed)

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Displaying 1 - 25 of 26

Variant Allele Characterization in STR Markers Using Next Generation Sequencing

May 29, 2026
Author(s)
Lauren Mullen, Carolyn Steffen, Katherine Gettings, Kevin Kiesler, Peter Vallone
Traditionally, Sanger sequencing was used to characterize reference materials and confirm discordant allele calls from different STR typing kits at the National Institute of Standards and Technology (NIST). Sequencing can also identify genomic variations

Advancing forensic SNP typing: Insights from an interlaboratory study of the FORCE panel

April 21, 2026
Author(s)
Andreas Tillmar, Kimberly Andreaggi, Adam Staadig, Christina Amory, David Ballard, Erik Francisco Bergseth, Claus Borsting, Selena Cisana, Michael Coble, Lucinda Bella Davenport, Maria de la Puente, Katherine Gettings, Erin Gorden, Kelly Grisedale, Jennifer Daniels-Higgenbotham, Rachel Houston, Sheree HUGHES, Reet Jarving, Kevin Kiesler, Jonathan King, Maria Victoria Lareu, Odile Loreille, Dennis McNevin, Melissa Muenzler, Walther Parson, Vania Pereira, Christopher Phillips, Jorge Ruiz-Ramirez, Maarja Sadam, Jennifer Snedeker, Carolyn Steffen, Monika Stoljarova-Bibb, Peter Vallone, Jodie Ward, Jessica Watson, Catarina Xavier, Daniel Kling, Charla Marshall
This study evaluated the ability to produce FORensic Capture Enrichment (FORCE) genotypes using amplicon-based and capture-based enrichment assays. Fifteen laboratories from three different continents participated in this study, choosing from one of four

Development of Publicly Available Forensic DNA Sequence Mixture Data

March 12, 2025
Author(s)
Erica Romsos, Kevin Kiesler, Carolyn Steffen, Lisa Borsuk, Sarah Riman, Lauren Mullen, Jodi Irwin, Peter Vallone, Katherine Gettings
Background: In 2018, the Next-Generation Sequencing Committee of SWGDAM queried bioinformatic and statistical interpretation method developers regarding data needs for the development of sequence-based probabilistic genotyping software. Methods: Based on

Review of SNP assays for DVI: cost, time, and performance information for decision-makers

July 17, 2024
Author(s)
Katherine Gettings, Andreas Tillmar, Kimberly Sturk-Andreaggi, Charla Marshall
In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan

Harmonizing the forensic nomenclature for STR Loci D6S474 and DYS612

January 21, 2024
Author(s)
Martin Bodner, David Ballard, Lisa Borsuk, Jonathan King, Walther Parson, Christopher Phillips, Katherine Gettings
The autosomal short tandem repeat (STR) locus D6S474 and the Y-chromosomal STR locus DYS612 have been reported multiple ways in the forensic literature, with differences in both the bracketed repeat structures and counting of numerical length-based

U.S. population data for 94 identity informative SNP loci

May 15, 2023
Author(s)
Kevin Kiesler, Lisa Borsuk, Becky Steffen, Peter Vallone, Katherine Gettings
The U.S. National Institute of Standards and Technology analyzed a set of 1036 samples representing four major U.S. population groups (African American, Asian American, Caucasian, and Hispanic) with the ForenSeq DNA Signature Prep Kit, DNA primer mix B

MAKE IT "SNPPY" - UPDATES TO SRM 2391D: PCR-BASED DNA PROFILING STANDARD

December 16, 2022
Author(s)
Becky Steffen, Erica Romsos, Kevin Kiesler, Lisa Borsuk, Katherine Gettings, Peter Vallone
Standard Reference Material (SRM) 2391d: PCR-Based DNA Profiling Standard was released to the forensic community in 2019. Next Generation Sequencing (NGS) was used as the primary method of certification, where certified values were assigned when a high

STRSeq: FAQ for submitting

December 1, 2022
Author(s)
Lisa Borsuk, Peter Vallone, Katherine Gettings
The STR sequencing project was developed due to the necessity of publicly sharing sequencing information about Short Tandem Repeats (STR) associated with human identification. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) is

Population data for 94 identity SNPs in four U.S. population groups

September 23, 2022
Author(s)
Kevin Kiesler, Lisa Borsuk, Becky Steffen, Katherine Gettings, Peter Vallone
The U.S. National Institute of Standards and Technology (NIST) sequenced 1036 human DNA samples from four United States population groups (African American, Asian, Hispanic, and Caucasian) using the ForenSeq DNA Signature Prep Kit with Primer Mix B (DPMB)

Sequence-based U.S. population data for 7 X STR loci

April 26, 2021
Author(s)
Lisa Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone, Katherine Gettings
The National Institute of Standards and Technology (NIST) U.S. population sample set of unrelated individuals was used to determine allele and haplotype frequencies for seven X-chromosome short tandem repeat (STR) loci in four linkage groups. DXS7132

Classification of STR allelic variation using massively parallel sequencing and assessment of flanking region power

July 12, 2020
Author(s)
Laurence Devesse, Lucinda Davenport, Lisa Borsuk, Katherine Gettings, Gabriella Mason-Buck, Peter Vallone, David Ballard
The application of massively parallel sequencing (MPS) to forensic genetics has led to improvements in multiple aspects of DNA analysis, however, additional complexities are concurrently associated with these advances. In relation to short tandem repeat

Report from the STRAND Working Group on the 2019 STR Sequence Nomenclature Meeting

September 21, 2019
Author(s)
Katherine Gettings, David Ballard, Martin Bodner, Lisa Borsuk, Jonathan King, Walther Parson, Christopher Phillips
This report summarizes topics discussed at the STR sequence nomenclature meeting hosted by the STRAND Working Group in April 2019. Invited attendees for this meeting included researchers known-to-us to be developing STR sequence-based nomenclature schemata

Sequence-based U.S. population data for 27 autosomal STR loci

July 19, 2018
Author(s)
Katherine Gettings, Lisa Borsuk, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone
This manuscript reports STR sequence-based allele frequencies for the "NIST 1036" sample set across 27 autosomal STR loci: D1S1656, TPOX, D2S441, D2S1338, D3S1358, D4S2408, FGA, D5S818, CSF1PO, D6S1043, D7S820, D8S1179, D9S1122, D10S1248, TH01, vWA

Sequence-based U.S. population data for the SE33 locus

May 7, 2018
Author(s)
Lisa Borsuk, Katherine Gettings, Carolyn R. Steffen, Kevin M. Kiesler, Peter Vallone
A set of 1036 U.S. Population Samples were sequenced using the Illumina ForenSeq DNA Signature Prep Kit. This sample set has been highly characterized using a variety of marker systems for human identification. The FASTQ files obtained from a ForenSeq DNA

"The devil's in the detail": Release of an expanded, enhanced and dynamically revised forensic STR Sequence Guide

February 21, 2018
Author(s)
Christopher Phillips, Katherine Gettings, Jonathan King, David Ballard, Martin Bodner, Lisa Borsuk, Walther Parson
The STR sequence template file published in 2016 as part of the considerations from the DNA Commission of theInternational Society for Forensic Genetics on minimal STR sequence nomenclature requirements, has been comprehensively revised and audited using

STRSeq: A catalog of sequence diversity at human identification Short Tandem Repeat loci

November 1, 2017
Author(s)
Katherine Gettings, Lisa Borsuk, D Ballard, Martin Bodner, Bruce Budowle, Laurence Devesse, Jonathan King, W. Parson, Christopher Phillips, Peter Vallone
The STR Sequencing Project (STRSeq) was initiated to facilitate the description of sequence- based alleles at the Short Tandem Repeat (STR) loci targeted in human identification assays. This international collaborative effort, which has been endorsed by

Sequence variation of 22 autosomal STR loci detected by next generation sequencing

December 1, 2015
Author(s)
Katherine Gettings, Kevin M. Kiesler, Seth A. Faith, Elizabeth Montano, Christine H. Baker, Brian A. Young, Richard A. Guerreri, Peter Vallone
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories

Sequence-based Analysis of Stutter at STR Loci: Characterization and Utility

September 23, 2015
Author(s)
Rachel A. Aponte, Katherine Gettings, David L. Duewer, Michael D. Coble, Peter Vallone
The development of next generation sequencing (NGS) technologies creates the potential for changing the method by which the forensic science community genotypes short tandem repeat (STR) loci. While the capabilities of NGS are promising, moving from

STR Allele Sequence Variation: Current Knowledge and Future Issues

July 6, 2015
Author(s)
Katherine Gettings, Rachel A. Aponte, Peter Vallone, John M. Butler
This article reviews what is currently known about short tandem repeat (STR) allelic sequence variation in and around the twenty-four loci most commonly used throughout the world to perform forensic DNA investigations. These STR loci include D1S1656, TPOX
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