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Search Publications by: Erica Romsos (Fed)

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Displaying 1 - 17 of 17

Rapid Production and Free Distribution of a Synthetic RNA Material to Support SARS-CoV-2 Molecular Diagnostic Testing

May 3, 2023
Megan Cleveland, Erica Romsos, Becky Steffen, Nathanael David Olson, Stephanie Servetas, William Valiant, Peter Vallone
In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This


December 16, 2022
Becky Steffen, Erica Romsos, Kevin Kiesler, Lisa Borsuk, Katherine Gettings, Peter Vallone
Standard Reference Material (SRM) 2391d: PCR-Based DNA Profiling Standard was released to the forensic community in 2019. Next Generation Sequencing (NGS) was used as the primary method of certification, where certified values were assigned when a high

Reference Material 8376 Microbial Pathogen DNA Standards for Detection and Identification

June 1, 2022
Jason Kralj, Dieter Tourlousse, Monique Hunter, Erica Romsos, Blaza Toman, Peter Vallone, Scott Jackson
Reference Material (RM) 8376 is intended for NGS-based measurements quantitative to the chromosome. A unit of RM 8376 consists of 20 components (A-T, 19 bacteria and 1 human) each containing well-characterized DNA in 10 mmol/L Tris-HCl, 1 mmol/L EDTA pH 8

Results of the 2018 Rapid DNA Maturity Assessment

January 27, 2020
Erica L. Romsos, Peter Vallone
Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in the July of 2018. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the

2018 Rapid DNA Maturity Assessment Results

September 26, 2018
Erica L. Romsos, Peter Vallone
The Rapid DNA Act, which amends the DNA Identification Act of 1994, allows for the integration of rapid DNA instruments for use by law enforcement for DNA testing of arrestees in a booking station environment. Several parallel efforts have been made to

Certification of Standard Reference Material(R) 2372a Human DNA Quantitation Standard

March 19, 2018
Erica L. Romsos, Margaret C. Kline, Natalia Farkas, David L. Duewer, Blaza Toman
Standard Reference Material (SRM) 2372a is intended for use in the value assignment of human genomic deoxyribonucleic acid (DNA) forensic quantitation materials. A unit of SRM 2372a consists of three well-characterized human genomic DNA materials

Rapid DNA Maturity Assessment

September 10, 2015
Erica L. Romsos, Sanae Lembirick, Peter Vallone
Two fully integrated rapid DNA platforms were tested as a part of a rapid DNA maturity assessment in the fall of 2014. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the current rapid DNA

Rapid PCR of STR Markers: Applications to Human Identification

April 23, 2015
Peter Vallone, Erica Romsos
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection

Rapid PCR Protocols for Forensic DNA Typing on Six Thermal Cycling Platforms

August 22, 2014
Peter Vallone, Erica Romsos
Rapid PCR protocols for the amplification of typing short tandem repeat multiplexes were evaluated on 6 different thermal cyclers. PCR primers from the commercially available multiplex short tandem repeat typing kit Identifiler were used to target 15 STR

The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c

November 1, 2011
Margaret C. Kline, Carolyn R. Steffen, Jamie L. Almeida, Erica L. Romsos, Michael D. Coble, John M. Butler
Standard Reference Material 2391c (SRM 2391c) PCR-based DNA Profiling Standard is the third renewal of this SRM, originally released in 1995. SRM 2391c consists of 6 candidate components labeled A through F. Components A through D are supplied as genomic