Certification of Standard Reference Material® 2372a Human DNA Quantitation Standard

Published

March 19, 2018

Author(s)

Erica L. Romsos, Margaret C. Kline, Natalia Farkas, David L. Duewer, Blaza Toman

Abstract

Standard Reference Material (SRM) 2372a is intended for use in the value assignment of human genomic deoxyribonucleic acid (DNA) forensic quantitation materials. A unit of SRM 2372a consists of three well-characterized human genomic DNA materials solubilized in 10 mmol/L 2- amino-2-(hydroxymethyl)-1,3 propanediol hydrochloride (Tris HCl) and 0.1 mmol/L thylenediaminetetraacetic acid disodium salt (disodium EDTA) pH 8.0 buffer (TE 4). The three component genomic DNA materials, labeled A, B, and C, are respectively derived from a single male donor, a single female donor, and 1:3 mixture of a male and a female donor. A unit of the SRM consists of one 0.5 mL tube of each component, each tube containing approximately 55 µL of DNA solution. Each of these tubes is labeled and is sealed with a color coded screw cap. This publication documents the production, analytical methods, and statistical evaluations involved in production of this SRM.

Citation

Special Publication (NIST SP) - 260-189

Report Number

260-189

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

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DOI Link

Keywords

Human mitochondrial DNA (mtDNA), Human nuclear DNA (nDNA), Standard Reference Material (SRM)

Citation

Romsos, E. , Kline, M. , Farkas, N. , Duewer, D. and Toman, B. (2018), Certification of Standard Reference Material® 2372a Human DNA Quantitation Standard, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.260-189 (Accessed April 19, 2024)