Three commercially available integrated rapid DNA instruments were tested as a part of a rapid DNA maturity assessment in the July of 2018. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the current rapid DNA typing technology. Samples were provided to participants for testing on the individual rapid platforms within their laboratories, and data was returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both FBI-defined automated (Rapid DNA Analysis) and manual (Modified Rapid DNA Analysis) review of the data sets were conducted to assess the success of genotyping the 20 CODIS core STR loci and full profiles generated by the instruments. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistry was combined into a single analysis. The assessment of the maturity of rapid DNA technology was focused on genotyping success of the 20 CODIS core STR loci and the total number of loci attempted per chemistry run. An analysis of the peak height ratios and basepair sizing precision across three platforms and two STR kit chemistries was performed.