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Rapid DNA Maturity Assessment

Published

Author(s)

Erica L. Romsos, Sanae Lembirick, Peter Vallone

Abstract

Two fully integrated rapid DNA platforms were tested as a part of a rapid DNA maturity assessment in the fall of 2014. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the current rapid DNA typing technology. Samples were provided to participants for testing on the individual rapid platforms, and data was returned to the National Institute of Standards and Technology (NIST) for review and analysis. Both automated and manual review of the data sets were conducted to assess the success of genotyping the CODIS 13 core STR loci. Genotype results from the multiple platforms, participating laboratories, and STR typing chemistry was combined into a single analysis. The current assessment of the maturity of rapid DNA technology was focused on genotyping success, peak height ratios, and stutter artifacts across two platforms and multiple STR kit chemistries.
Citation
Forensic Science International: Genetics Supplement Series
Volume
5

Keywords

Rapid DNA, Integrated Device, Rapid PCR

Citation

Romsos, E. , Lembirick, S. and Vallone, P. (2015), Rapid DNA Maturity Assessment, Forensic Science International: Genetics Supplement Series, [online], https://doi.org/10.1016/j.fsigss.2015.09.001, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=919621 (Accessed April 23, 2024)
Created September 9, 2015, Updated November 10, 2018