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2018 Rapid DNA Maturity Assessment Results

Published

Author(s)

Erica L. Romsos, Peter M. Vallone

Abstract

The Rapid DNA Act, which amends the DNA Identification Act of 1994, allows for the integration of rapid DNA instruments for use by law enforcement for DNA testing of arrestees in a booking station environment. Several parallel efforts have been made to integrate the forensic STR analysis workflow and utilize a simple swab in, answer out process within a single platform. Of the multiple efforts, three platforms were included as a part of a rapid DNA maturity assessment. The assessment was conducted with sets of 20 blinded single-source reference samples to examine the success of the current rapid DNA typing technology, specifically for the 20 CODIS core STR loci. Samples were provided to participants for testing on their rapid platform of choice. Electropherograms and corresponding allele calls were returned to the National Institute of Standards and Technology (NIST) for review and further analysis. Both automated and manual review of the data sets were conducted to assess the success of typing the 20 CODIS core loci. Genotyping profiles from the multiple platforms, participants, and STR typing chemistries were combined into a single analysis to assess the current maturity of Rapid DNA technology. The presented results will focus on the genotyping success rate for the 20 core markers, peak height ratio balance, and stutter artifacts across three platforms and STR kit chemistries.
Proceedings Title
ISHI Proceedings
Conference Dates
September 24-27, 2018
Conference Location
Phoenix, AZ
Conference Title
International Symposium on Human Identification (ISHI)

Keywords

forensic DNA, rapid DNA, human identification
Created September 26, 2018, Updated February 19, 2020