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The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c

Published

Author(s)

Margaret C. Kline, Carolyn R. Steffen, Jamie L. Almeida, Erica L. Romsos, Michael D. Coble, John M. Butler

Abstract

Standard Reference Material 2391c (SRM 2391c) PCR-based DNA Profiling Standard is the third renewal of this SRM, originally released in 1995. SRM 2391c consists of 6 candidate components labeled A through F. Components A through D are supplied as genomic DNA solutions packaged in perfluoroalkoxy fluoropolymer (PFA) vials (with component A as a single source female; component B as a single source male; component C as a single source male: and component D as a mixture of 3 parts components A and 1 part component C. Components E (single source female) and F (single source male) are cells (=75,000 cells per spot) deposited on 903 and FTA papers respectively, two 6 mm punches per component. Inclusion of two kinds of storage paper will enable laboratores to test direct PCR methods.
Citation
No Journal Selected

Citation

Kline, M. , Steffen, C. , Almeida, J. , Romsos, E. , Coble, M. and Butler, J. (2011), The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c, No Journal Selected (Accessed April 18, 2024)
Created November 1, 2011, Updated November 10, 2018