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Search Publications by: Michael Halter (Fed)

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Displaying 26 - 37 of 37

Quantitative Methods to Characterize Cell Lines: Comparison of Cells from Marine and Terrestrial Mammals.

July 28, 2012
Author(s)
Tighe Spurlin, John T. Elliott, Michael W. Halter, Kiran Bhadriraju, Alessandro Tona, Anne L. Plant, Annalaura Mancia, Bobby L. Middlebrooks, Gregory W. Warr
Descriptive terms are often used to characterize cells in culture, but the use of nonquantitative and poorly defined terms can lead to ambiguities when comparing data from different laboratories. Although recently there has been a good deal of interest in

A New Measure in Cell Image Segmentation Data Analysis

July 24, 2012
Author(s)
Jin Chu Wu, Michael W. Halter, Raghu N. Kacker, John T. Elliott
Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells treated as

Evaluation of Segmentation Algorithms on Cell Populations Using CDF Curves

February 24, 2012
Author(s)
Robert C. Hagwood, Javier Bernal, Michael W. Halter, John T. Elliott
Cell segmentation is a critical step in the analysis pipeline for most imaging cytometry experiments and the segmentation algorithm can effect the quantitative data derived from image analysis. Methods to evaluate segmentation algorithms are important for

Comparison of segmentation algorithms for fluorescence microscopy images of cells

June 14, 2011
Author(s)
Alden A. Dima, John T. Elliott, James J. Filliben, Michael W. Halter, Adele P. Peskin, Javier Bernal, Marcin Kociolek, Mary C. Brady, Hai C. Tang, Anne L. Plant
Segmentation results from nine different segmentation techniques applied to two different cell lines and five different sets of imaging conditions were compared. Significant variability in the results of segmentation was observed that was due solely to

Package "cellVolumeDist"

April 13, 2010
Author(s)
Katharine M. Mullen, Michael W. Halter
This package implements a methodology for using cell volume distributions to estimate cell growth rates and division times that is described in the paper entitled "Cell Volume Distributions Reveal Cell Growth Rates and Division Times", by Michael Halter

AN AUTOMATIC OVERLAP-BASED CELL TRACKING SYSTEM

February 26, 2010
Author(s)
Joe Chalfoun, Antonio Cardone, Alden A. Dima, Michael Halter, Daniel P. Allen
In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an overlap-based cell tracking algorithm that has the ability to track cells across a set of time-lapse

Overlap-Based Cell Tracker

February 2, 2010
Author(s)
Joe Chalfoun, Antonio Cardone, Alden A. Dima, Michael Halter, Daniel P. Allen
In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by NIST. This

A mechanistically relevant cytotoxicity assay based on the detection of cellular GFP

August 1, 2009
Author(s)
Michael W. Halter, Jamie L. Almeida, Alessandro Tona, Kenneth D. Cole, Anne L. Plant, John T. Elliott
Cell-based assays for measuring ribosome inhibition by proteins such as the plant toxin ricin are important for characterizing decontamination strategies and developing detection technologies for field use. We report here an assay for ricin that provides a

Cell volume distributions reveal cell growth rates and division times

March 7, 2009
Author(s)
Michael W. Halter, John T. Elliott, Joseph B. Hubbard, Alessandro Tona, Anne L. Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable rates

Surface plasmon resonance imaging of cells and surface-associated fibronectin

February 26, 2009
Author(s)
Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant
Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for