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Overlap-Based Cell Tracker



Joe Chalfoun, Antonio Cardone, Alden A. Dima, Michael Halter, Daniel P. Allen


In order to facilitate the extraction of quantitative data from live cell image sets, automated image analysis methods are needed. This paper presents an introduction to the general principle of an overlap cell tracking software developed by NIST. This cell tracker has the ability to track cells across a set of time lapse images acquired at high rates based on the amount of overlap between cellular regions in consecutive frames. It is designed to be highly flexible, requires little user parameterization, and has a fast execution time.
NIST Interagency/Internal Report (NISTIR) - 7663
Report Number


overlap-based cell tracking, time-lapse cell imaging, cell motility, live-cell imaging


Chalfoun, J. , Cardone, A. , Dima, A. , Halter, M. and Allen, D. (2010), Overlap-Based Cell Tracker, NIST Interagency/Internal Report (NISTIR), National Institute of Standards and Technology, Gaithersburg, MD, [online], (Accessed May 26, 2024)


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Created February 1, 2010, Updated October 12, 2021