Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Surface plasmon resonance imaging of cells and surface-associated fibronectin

Published

Author(s)

Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant

Abstract

Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for examining cell-matrix interactions. A label-free imaging method would provide an alternative that would eliminate the requirement of transfected cells and modified biological molecules, and if collected nondestructively, would allow long term observation and analysis of live cells. Results Using surface plasmon resonance imaging (SPRI), the deposition of protein by vascular smooth muscle cells (vSMC) cultured on fibronectin was quantified as a function of cell density and distance from the cell periphery. We observed that as much as 120 ng/cm2 of protein was deposited by cells in 24 h. Conclusion SPRI is a real-time, low-light-level, label-free imaging technique that allows the simultaneous observation and quantification of protein layers and cellular features. This technique is compatible with live cells such that it is possible to monitor cellular modifications to the extracellular matrix in real-time.
Citation
BMC Cell Biology
Volume
10

Keywords

vascular smooth muscle cells, extracellular matrix, fibronectin, imaging, image analysis, label-free, surface plasmon

Citation

Peterson, A. , Halter, M. , Tona, A. , Bhadriraju, K. and Plant, A. (2009), Surface plasmon resonance imaging of cells and surface-associated fibronectin, BMC Cell Biology, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=902594 (Accessed July 6, 2022)
Created February 26, 2009, Updated February 19, 2017