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Search Publications by: Peter M. Vallone (Fed)

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Displaying 76 - 100 of 103

The evaluation of an autosomal SNP 12-plex assay

April 1, 2006
Author(s)
Peter M. Vallone, Amy E. Decker, Michael D. Coble, John M. Butler
SNPs have the potential to play a useful role in human identification testing. Small PCR amplicon sizes associated with SNP typing technologies make SNPs attractive for typing degraded DNA or other low copy number situations. SNP markers can be useful in

Analysis of DNA Single Nucleotide Polymorphisms by Mass Spectrometry

March 17, 2006
Author(s)
Peter Vallone, John Butler
Single nucleotide polymorphisms(SNP) are the most frequent form of DNA sequence variation in the human genome and are becoming increasingly useful as genetic markers for genome mapping studies, medical diagnostics, and human identity testing. The primer

Effective Strategies for Forensic Analysis in the Mitochondrial DNA Coding Region

January 1, 2006
Author(s)
Michael D. Coble, Peter Vallone, Rebecca S. Just, Toni M. Diegoli, Brion C. Smith, T. J. Parsons
Recently, it has been recognized that accessing information in the mitochondrial DNA (mtDNA) coding region can provide additional forensic discrimination with respect to the standard typing of the D-loop region, augmenting the sometimes rather limited

Genotyping SNPs Using a UV Photocleavable Oligonucleotide in MALDI-TOF MS

March 17, 2005
Author(s)
Peter M. Vallone, K Fahr, M Kostrzewa
Matrix assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) coupled with allele specific primer extension is a proven method for typing single nucleotide polymorphisms (SNPs). A novel modification upon this methodology is the

Mitochondrial DNA Typing Screens With Control Region and Coding Region SNPs

March 1, 2005
Author(s)
Margaret C. Kline, Peter M. Vallone, Janette W. Redman, David L. Duewer, C D. Calloway, John M. Butler
Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. The

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers

September 10, 2002
Author(s)
John M. Butler, R Schoske, Peter M. Vallone, Margaret C. Kline, A J. Redd, M F. Hammer
A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extended

General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers

January 1, 2002
Author(s)
R Schoske, Peter Vallone, C M. Ruitberg, John Butler
The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PCR

Development of Y STR Megaplex Assays

December 1, 2001
Author(s)
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001
Author(s)
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

February 1, 2001
Author(s)
John M. Butler, C M. Ruitberg, Peter M. Vallone
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining
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