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Search Publications by: Peter M. Vallone (Fed)

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Displaying 101 - 125 of 186

Rapid PCR of STR Markers: Applications to Human Identification

April 23, 2015
Author(s)
Peter Vallone, Erica Romsos
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection

Rapid PCR Protocols for Forensic DNA Typing on Six Thermal Cycling Platforms

August 22, 2014
Author(s)
Peter Vallone, Erica Romsos
Rapid PCR protocols for the amplification of typing short tandem repeat multiplexes were evaluated on 6 different thermal cyclers. PCR primers from the commercially available multiplex short tandem repeat typing kit Identifiler were used to target 15 STR

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

January 1, 2013
Author(s)
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone, A. Carracedo
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

Evaluating self-declared ancestry of U.S. Americans using autosomal, Y-chromosomal and mitochondrial DNA

December 31, 2010
Author(s)
Peter Vallone, Oscar Leo, Manfred Kayser, Mannis van Oven, Michael Coble, Toni M. Diegoli, Kristiaan J. van der Gaag, Peter D. Knijff
The current U.S. population represents an amalgam of individuals from different biogeographic ancestries who arrived on the territory at different times in the history of the territory. However, such admixture is far from being homogeneous and this fact

A New 26plex Autosomal STR Assay to Aid Human Identity Testing*(dagger)

September 1, 2009
Author(s)
Carolyn R. Steffen, John M. Butler, Peter Vallone
Multiplex polymerase chain reaction (PCR) has become the standard in forensic testing. Currently there are two commercial multiplex PCR amplification kits available that simultaneously amplify 16 short tandem repeat (STR) loci that include the 13 FBI

Demonstration of Rapid Multiplex PCR Amplification Involving 16 Genetic Loci

December 1, 2008
Author(s)
Peter Vallone, Carolyn R. Steffen, John M. Butler
Current forensic DNA typing is conducted in approximately eight to ten hours with steps including DNA extraction, quantitation, polymerase chain reaction (PCR) amplification of multiple short tandem repeat (STR) loci, capillary electrophoresis separation

An Investigation of Discrimination Capacity and the Cause of Null Alleles in Linear Array Mitostrips Using Control Region Sequence Data.

October 16, 2008
Author(s)
Michael D. Coble, Margaret C. Kline, Janette W. Redman, Amy E. Decker, Peter Vallone, John Butler
Mitochondrial DNA (mtDNA) analysis of forensic evidentiary materials such as degraded bones and shed hairs can provide the forensic scientist with some genetic information especially when highly discriminatory systems, such as nuclear STRs, completely fail

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Author(s)
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Analysis of artificially degraded DNA using STRs and SNPs results of a collaborative European (EDNAP) exercise

December 1, 2006
Author(s)
L. A. Dixon, A. E. Dobbins, H. Pulker, John M. Butler, Peter M. Vallone, Michael D. Coble, W. Parson, B. Berger, P. Grubwieser, H. S. Mogensen, N. Morling, K. Nielsen, J. J. Sanchez, E. Petkovski, A. Carracedo, P. Sanchez-Diz, E. Ramos-Luis, M. Brion, J. A. Irwin, R. S. Just, O. Loreille, T. J. Parsons, D. Syndercombe-Court, H. Schmitter, B. Stradmann-Bellinghausen, K. Bender, P. Gill
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