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Search Publications by: Peter M. Vallone (Fed)

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Displaying 51 - 75 of 103

Sequence variation of 22 autosomal STR loci detected by next generation sequencing

December 1, 2015
Author(s)
Katherine Gettings, Kevin M. Kiesler, Seth A. Faith, Elizabeth Montano, Christine H. Baker, Brian A. Young, Richard A. Guerreri, Peter Vallone
Sequencing short tandem repeat (STR) loci allows for determination of repeat motif variations within the STR (or entire PCR amplicon) which cannot be ascertained by size-based PCR fragment analysis. Sanger sequencing has been used in research laboratories

Sequence-based Analysis of Stutter at STR Loci: Characterization and Utility

September 23, 2015
Author(s)
Rachel A. Aponte, Katherine Gettings, David L. Duewer, Michael D. Coble, Peter Vallone
The development of next generation sequencing (NGS) technologies creates the potential for changing the method by which the forensic science community genotypes short tandem repeat (STR) loci. While the capabilities of NGS are promising, moving from

Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard

September 16, 2015
Author(s)
Carolyn R. Steffen, Margaret C. Kline, David L. Duewer, Peter Vallone
The NIST Standard Reference Material (SRM) 2391c: PCR-Based DNA Profiling Standard was updated in April 2015 to contain new information relevant to the forensic community. Previously, there were certified genotypes for 24 autosomal STR markers plus

Rapid DNA Maturity Assessment

September 10, 2015
Author(s)
Erica L. Romsos, Sanae Lembirick, Peter Vallone
Two fully integrated rapid DNA platforms were tested as a part of a rapid DNA maturity assessment in the fall of 2014. The assessment was conducted with sets of blinded single-source reference samples to gauge the typing success of the current rapid DNA

STR Allele Sequence Variation: Current Knowledge and Future Issues

July 6, 2015
Author(s)
Katherine Gettings, Rachel A. Aponte, Peter Vallone, John M. Butler
This article reviews what is currently known about short tandem repeat (STR) allelic sequence variation in and around the twenty-four loci most commonly used throughout the world to perform forensic DNA investigations. These STR loci include D1S1656, TPOX

Performance of a next generation sequencing SNP assay on degraded DNA

May 27, 2015
Author(s)
Katherine Gettings, Kevin M. Kiesler, Peter Vallone
Forensic DNA casework samples are often of insufficient quantity or quality to generate full profiles by conventional DNA typing methods. Amplification of STR loci is inherently limited in samples containing degraded DNA, as the cumulative size of repeat

Rapid PCR of STR Markers: Applications to Human Identification

April 23, 2015
Author(s)
Peter Vallone, Erica Romsos
Multiplex PCR with fluorescently labeled primers has been an essential method for the amplification of short tandem repeats used in human identify testing. Within the STR workflow of extraction, quantitation, amplification, separation, and detection

Rapid PCR Protocols for Forensic DNA Typing on Six Thermal Cycling Platforms

August 22, 2014
Author(s)
Peter Vallone, Erica Romsos
Rapid PCR protocols for the amplification of typing short tandem repeat multiplexes were evaluated on 6 different thermal cyclers. PCR primers from the commercially available multiplex short tandem repeat typing kit Identifiler were used to target 15 STR

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

January 1, 2013
Author(s)
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone, A. Carracedo
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

Evaluating self-declared ancestry of U.S. Americans using autosomal, Y-chromosomal and mitochondrial DNA

December 31, 2010
Author(s)
Peter Vallone, Oscar Leo, Manfred Kayser, Mannis van Oven, Michael Coble, Toni M. Diegoli, Kristiaan J. van der Gaag, Peter D. Knijff
The current U.S. population represents an amalgam of individuals from different biogeographic ancestries who arrived on the territory at different times in the history of the territory. However, such admixture is far from being homogeneous and this fact

A New 26plex Autosomal STR Assay to Aid Human Identity Testing*(dagger)

September 1, 2009
Author(s)
Carolyn R. Steffen, John M. Butler, Peter Vallone
Multiplex polymerase chain reaction (PCR) has become the standard in forensic testing. Currently there are two commercial multiplex PCR amplification kits available that simultaneously amplify 16 short tandem repeat (STR) loci that include the 13 FBI

Demonstration of Rapid Multiplex PCR Amplification Involving 16 Genetic Loci

December 1, 2008
Author(s)
Peter Vallone, Carolyn R. Steffen, John M. Butler
Current forensic DNA typing is conducted in approximately eight to ten hours with steps including DNA extraction, quantitation, polymerase chain reaction (PCR) amplification of multiple short tandem repeat (STR) loci, capillary electrophoresis separation

An Investigation of Discrimination Capacity and the Cause of Null Alleles in Linear Array Mitostrips Using Control Region Sequence Data.

October 16, 2008
Author(s)
Michael D. Coble, Margaret C. Kline, Janette W. Redman, Amy E. Decker, Peter Vallone, John Butler
Mitochondrial DNA (mtDNA) analysis of forensic evidentiary materials such as degraded bones and shed hairs can provide the forensic scientist with some genetic information especially when highly discriminatory systems, such as nuclear STRs, completely fail

Microfluidic DNA Analysis Systems for Forensic Applications

July 18, 2008
Author(s)
Michael Gaitan, Jayna J. Shah, Darwin Reyes-Hernandez, Pierre-Alain Auroux, Jon Geist, Laurie E. Locascio, Wyatt N. Vreeland, David J. Ross, Peter Vallone, Paul Smith, Nicole Morgan, Tom Pohida, John Kakareka, Annelise Barron
This report summarizes the NIST effort on microfluidic DNA analysis systems for forensic applications sponsored by the National Institute of Justice. Currently emerging microfluidics-based forensic systems are implemented in silica (glass) because the

Analysis of artificially degraded DNA using STRs and SNPs results of a collaborative European (EDNAP) exercise

December 1, 2006
Author(s)
L. A. Dixon, A. E. Dobbins, H. Pulker, John M. Butler, Peter M. Vallone, Michael D. Coble, W. Parson, B. Berger, P. Grubwieser, H. S. Mogensen, N. Morling, K. Nielsen, J. J. Sanchez, E. Petkovski, A. Carracedo, P. Sanchez-Diz, E. Ramos-Luis, M. Brion, J. A. Irwin, R. S. Just, O. Loreille, T. J. Parsons, D. Syndercombe-Court, H. Schmitter, B. Stradmann-Bellinghausen, K. Bender, P. Gill

Setting standards and developing technology to aid the human identity testing community

April 1, 2006
Author(s)
John M. Butler, Michael D. Coble, Amy E. Decker, David L. Duewer, Carolyn R. Steffen, Margaret C. Kline, Janette W. Redman, Peter Vallone
Our project team at the U.S. National Institute of Standards and Technology (NIST) is funded by the National Institute of Justice (NIJ) to conduct research that benefits the human identity testing community and to create tools that enable forensic DNA
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