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Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard



Carolyn R. Steffen, Margaret C. Kline, David L. Duewer, Peter Vallone


The NIST Standard Reference Material (SRM) 2391c: PCR-Based DNA Profiling Standard was updated in April 2015 to contain new information relevant to the forensic community. Previously, there were certified genotypes for 24 autosomal STR markers plus Amelogenin and 17 Y-STR markers. Due to the increase in markers present in larger commercial autosomal STR and Y-STR multiplex kits recently released, there is a need to add certified types for these new markers for each component of SRM 2391c (Components A-F). The updated Certificate of Analysis has certified values for 1 additional autosomal STR marker (D6S1043) and 12 additional Y-STR markers (29 total). Also, the number of STR multiplex assays tested increased from 24 to 43. Sanger sequencing was performed on Components A-C, E and F (Component D is a mixture of Components A and C) for all the certified markers to determine the STR repeat motifs and to characterize adjacent flanking regions and underlying polymorphisms (sequence, insertion-deletion, variation in complex motifs) typically not detected by fragment-based typing. SRM 2391c Components A-F can be used to establish traceability in a laboratory based on the updated certified and reference genotypes/haplotypes (information values cannot be used for NIST traceable materials). The simple steps to achieve NIST traceability with SRM 2391c will be explained.
Forensic Science International: Genetics Supplement Series


DNA sequencing, standard reference material, short tandem repeat, DNA typing, Sanger sequencing, traceable material


Steffen, C. , Kline, M. , Duewer, D. and Vallone, P. (2015), Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard, Forensic Science International: Genetics Supplement Series, [online], (Accessed June 20, 2024)


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Created September 15, 2015, Updated November 10, 2018