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Search Publications by: Peter M. Vallone (Fed)

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Displaying 76 - 88 of 88

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers

September 10, 2002
Author(s)
John M. Butler, R Schoske, Peter M. Vallone, Margaret C. Kline, A J. Redd, M F. Hammer
A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extended

General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers

January 1, 2002
Author(s)
R Schoske, Peter Vallone, C M. Ruitberg, John Butler
The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PCR

Development of Y STR Megaplex Assays

December 1, 2001
Author(s)
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001
Author(s)
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

February 1, 2001
Author(s)
John M. Butler, C M. Ruitberg, Peter M. Vallone
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining

DNA Mass Spectrometry at NIST

February 1, 2001
Author(s)
Peter M. Vallone
The use of mass spectrometry as a tool for solving biochemical problems is growing. Specifically, matrix assisted laser desorption ionization coupled with time-of-flight detection systems (MALDI-TOF) is emerging for high throughput and highaccuracy

Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA

February 1, 2000
Author(s)
Peter M. Vallone, R. Owczarzy, P. V. Riccelli, A. S. Benight
Melting Analysis of DNA Dumbbells with Repeating Dinucleotide Stem Sequences Reveals Evidence for Long Range Sequence Dependent Interactions in Short Duplex DNA. Optical and DSC melting studies were performed on 12 DNAdumbbells in 85 mM Na+. The stems of