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Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

Published

Author(s)

John M. Butler, C M. Ruitberg, Peter M. Vallone

Abstract

Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling condidtions. The process of optimizing a multiplex PCR reaction in order to obtain a well-balanced set of amplicons can be time-consuming and labor-intensive. The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process.
Citation
Fresenius Journal of Analytical Chemistry
Volume
369
Issue
No. 3-4

Keywords

DNA molecule, polymerase chain reaction (PCR)

Citation

Butler, J. , Ruitberg, C. and Vallone, P. (2001), Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions, Fresenius Journal of Analytical Chemistry (Accessed March 5, 2024)
Created February 1, 2001, Updated February 17, 2017