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Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions



John M. Butler, C M. Ruitberg, Peter M. Vallone


Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining various combinations of those primer sets and different reaction components and/or thermal cycling condidtions. The process of optimizing a multiplex PCR reaction in order to obtain a well-balanced set of amplicons can be time-consuming and labor-intensive. The rapid separation and quantitation capabilities of capillary electrophoresis make it an efficient technique to help in the multiplex PCR optimization process.
Fresenius Journal of Analytical Chemistry
No. 3-4


DNA molecule, polymerase chain reaction (PCR)


Butler, J. , Ruitberg, C. and Vallone, P. (2001), Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions, Fresenius Journal of Analytical Chemistry (Accessed March 5, 2024)
Created February 1, 2001, Updated February 17, 2017