Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification reactions where 8 or more STR loci are simultaneously copied. Commercially available kits are now widely used for STR amplification and subsequent DNA typing. We present here the use of high performance liquid chromatography (HPLC) and time-of-flight mass spectrometry (TOF-MS) methods for characterization of commercially available STR kits. The STR kits evaluated include AmpFlSTR Profiler , Profiler Plus , COfiler , and SGM Plus from PE Biosystems and GenePrint PowerPlex 1.1 and 2.1 from Promega Corporation.
Forensic Science International
HPLC, mass spectrometry, multiplex PCR amplification, oligonacleotide quality control, short tanden repeat DNA typing