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Displaying 26 - 50 of 70

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1997 Comparability Studies: Results for Round Robin XXXIX, XL, and XLI Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 10 Ascorbic Acid in Human Serum

August 7, 2013
Author(s)
David L. Duewer, Margaret C. Kline, Katherine E. Sharpless, Jeanice M. Brown Thomas, Sam A. Margolis
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1998 Comparability Studies: Results for Round Robin XLII, XLIII, and XLIV Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 11 Ascorbic Acid in Human Seru

August 7, 2013
Author(s)
David L. Duewer, Margaret C. Kline, Katherine E. Sharpless, Jeanice M. Brown Thomas, Sam A. Margolis
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Summer 2002 Comparability Studies: Results for Round Robin LII Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 17 Ascorbic Acid in Human Serum

June 27, 2013
Author(s)
David L. Duewer, Katherine E. Sharpless, Margaret C. Kline, Jeanice M. Brown Thomas, Sam A. Margolis
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

The Biological Evidence Preservation Handbook: Best Practices for Evidence Handlers

April 23, 2013
Author(s)
Susan M. Ballou, Margaret C. Kline, Mark D. Stolorow, Melissa Taylor, Shannan Williams, Phylis S. Bamberger, Burney Yvette, Larry Brown, Cynthia E. Jones, Ralph Keaton, William Kiley, Karen Thiessen, Gerry LaPorte, Joseph Latta, Linda E. Ledray, Randy Nagy, Linda Schwind, Stephanie Stoiloff, Brian Ostrom
The report of the Technical Working Group on Biological Evidence Preservation offers guidance for individuals involved in the collection, examination, tracking, packaging, storing, and disposition of biological evidence. This may include crime scene

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

March 1, 2013
Author(s)
Ross J. Haynes, Margaret C. Kline, Blaza Toman, Calum Scott, Paul Wallace, John M. Butler, Marcia J. Holden
Cytomegalovirus (CMV) is one of a number of human viruses that are ubiquitous in human populations, generally cause little illness upon infecting healthy individuals, but can cause life threatening disease in immune-compromised individuals. An important

Developmental Validation of the PowerPlex(R) ESI 17 Pro System

December 27, 2012
Author(s)
Carolyn R. Steffen, Margaret C. Kline, John Butler, Robert S. McLaren, Jaynish Patel, Margaret Ewing, Douglas R. Storts, Fabrice Noel, Sophie Dognaux
The SE33 locus is one of the most polymorphic markers used in human identification. However, it also possesses multiple microvariants both within the repeat and in the flanking regions. Such flanking region mutations can generate discordant allele calls

The Latest and Greatest NIST PCR-based DNA Profiling Standard: Updates and Status of Candidate Standard Reference Material (SRM) 2391c

November 1, 2011
Author(s)
Margaret C. Kline, Carolyn R. Steffen, Jamie L. Almeida, Erica L. Romsos, Michael D. Coble, John M. Butler
Standard Reference Material 2391c (SRM 2391c) PCR-based DNA Profiling Standard is the third renewal of this SRM, originally released in 1995. SRM 2391c consists of 6 candidate components labeled A through F. Components A through D are supplied as genomic

STR Sequence Analysis for Characterizing Normal, Variant, and Null Alleles

August 1, 2011
Author(s)
Margaret C. Kline, Carolyn R. Steffen, John M. Butler, Amy Decker
DNA sequence variation is known to exist in and around the repeat region of short tandem repeat (STR) loci used in human identity testing. While the vast majority of STR alleles measured in forensic DNA laboratories worldwide type as "normal" alleles

Metrology Needs and NIST Resources for the Forensic DNA Community

June 1, 2011
Author(s)
Michael D. Coble, Margaret C. Kline, John M. Butler
With the advent of Forensic DNA profiling in the mid-1980s, this technology has had a positive impact on the criminal justice system, helping to convict the guilty and exonerate the innocent. The field has evolved from focusing on multi-locus markers

Strategies for Concordance Testing

May 1, 2010
Author(s)
Carolyn R. Steffen, David L. Duewer, Margaret C. Kline, John M. Butler
Concordance evaluations are important to conduct to determine if there are any allelic dropout or “null alleles” present in a data set. These studies are based on the fact that there are a variety of commercial short tandem repeat (STR) multiplex kits

Addressing Y-Chromosome Short Tandem Repeat(Y-STR) Allele Nomenclature

November 25, 2008
Author(s)
John M. Butler, Margaret C. Kline, Amy E. Decker
Currently 120 different Y-chromosome short tandem repeat (Y-STR) markers are used by various genetic genealogy testing laboratories. In some cases, different laboratories may designate the same Y-STR allele with two different nomenclatures making data

An Investigation of Discrimination Capacity and the Cause of Null Alleles in Linear Array Mitostrips Using Control Region Sequence Data.

October 16, 2008
Author(s)
Michael D. Coble, Margaret C. Kline, Janette W. Redman, Amy E. Decker, Peter Vallone, John Butler
Mitochondrial DNA (mtDNA) analysis of forensic evidentiary materials such as degraded bones and shed hairs can provide the forensic scientist with some genetic information especially when highly discriminatory systems, such as nuclear STRs, completely fail

Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

January 25, 2008
Author(s)
Carolyn R. Steffen, Margaret C. Kline, Michael Coble, John M. Butler
An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized to aid in the analysis of degraded DNA samples. These new markers produce short PCR products in the target range of 50 150 base pairs (bp) by moving the

Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits

July 25, 2007
Author(s)
Carolyn R. Steffen, Margaret C. Kline, Julio J. Mulero, Robert E. Lagace, Chien-Wei Chang, Lori K. Hennessy, John M. Butler
The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA

Setting standards and developing technology to aid the human identity testing community

April 1, 2006
Author(s)
John M. Butler, Michael D. Coble, Amy E. Decker, David L. Duewer, Carolyn R. Steffen, Margaret C. Kline, Janette W. Redman, Peter Vallone
Our project team at the U.S. National Institute of Standards and Technology (NIST) is funded by the National Institute of Justice (NIJ) to conduct research that benefits the human identity testing community and to create tools that enable forensic DNA

Results From the NIST 2004 DNA Quantitation Study

May 1, 2005
Author(s)
Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler
For optimal DNA Short Tandem Repeat (STR) typing results, the DNA concentration ([DNA]) of the sample must be accurately determined prior to the polymerase chain reaction (PCR) amplification step in the typing process. In early 2004, the National Institute

Mitochondrial DNA Typing Screens With Control Region and Coding Region SNPs

March 1, 2005
Author(s)
Margaret C. Kline, Peter M. Vallone, Janette W. Redman, David L. Duewer, C D. Calloway, John M. Butler
Mitochondrial DNA (mtDNA) analysis has found an important niche in forensic DNA typing. It is used with highly degraded samples or low-copy number materials such as might be found from shed hair or bones exposed to severe environmental conditions. The