Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits

Published

July 25, 2007

Author(s)

Carolyn R. Steffen, Margaret C. Kline, Julio J. Mulero, Robert E. Lagace, Chien-Wei Chang, Lori K. Hennessy, John M. Butler

Abstract

The AmpFlSTR MiniFiler PCR Amplification kit developed by Applied Biosystems enables size reduction on eight of the larger short tandem repeat (STR) loci amplified in the Identifiler kit, which will aid recovery of information from highly degraded DNA samples. MiniFiler amplifies CSF1PO, FGA, D2S1338, D7S820, D13S317, D16S539, D18S51, and D21S11 as well as the sex-typing locus amelogenin. A total of 1,313 samples were evaluated with both the MiniFiler and Identifiler STR kits: 457 African American, 446 Caucasian, 206 Hispanic, and 204 Asian individuals. Full concordance between Identifiler and MiniFiler was observed in 99.7% (10,475 out of 10,504) STR allele calls compared. The 29 differences seen are listed in Table 1 and encompass the loci D13S317 (n=14) and D16S539 (n=9) as well as D18S51 (n=1), D7S820 (n=1), D2S1338 (n=3) and CSF1PO (n=1). Genotyping discrepancies between the Identifiler and MiniFiler kits were confirmed by re-amplification of the samples and further testing using the PowerPlex 16 kit in many cases. DNA sequence analysis was also performed in order to understand the nature of the genetic variations causing the allele dropout or apparent repeat unit shift.

Citation

Journal of Forensic Sciences

Volume

52

Issue

4

Pub Type

Journals

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Keywords

amelogenin, D16S539, D18S51, D21S11, D2S1338

Citation

Steffen, C. , Kline, M. , Mulero, J. , Lagace, R. , Chang, C. , Hennessy, L. and Butler, J. (2007), Concordance Study Between the AmpFlSTR((R)) MiniFiler(TM) PCR Amplification Kit and Conventional STR Typing Kits, Journal of Forensic Sciences, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=830531 (Accessed December 14, 2024)