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Search Publications

NIST Authors in Bold

Displaying 451 - 475 of 1242

Large-scale Time-lapse Microscopy of Oct4 Expression in Human Embryonic Stem Cell Colonies

May 22, 2016
Author(s)
Kiran Bhadriraju, Michael Halter, Julien M. Amelot, Peter Bajcsy, Joe Chalfoun, Antoine Vandecreme, Barbara Mallon, Kye-yoon Park, John T. Elliott, Subhash Sista, Anne L. Plant
Identification of critical characteristics of therapeutically active cells, and quantification of those characteristics, are essential for assuring consistency and potency of cell therapy products. We have developed image analysis and visualization

Comparison of T1 measurement using ISMRM/NIST system phantom

May 9, 2016
Author(s)
Kathryn E. Keenan, Karl F. Stupic, Michael A. Boss, Stephen E. Russek, Thomas L. Chenevert, Pottumarthi V. Prasad, Wilburn E. Reddick, Jie Zheng, Peng Hu, Edward F. Jackson
We used the ISMRM/NIST system phantom to assess variations of T1 measurements across MRI systems at 1.5 T and 3 T, to determine the repeatability and reproducibility of the T1 measurements. This study demonstrates that T1 variations from NMR-measured value

A Roadmap for Regulatory Science Research for Next Generation Sequencing Informatics

April 20, 2016
Author(s)
Justin M. Zook, Marc L. Salit, Russ B. Altman, Arend Sidow, Rachel Goldfeder, Euan Ashley, Elizabeth Mansfield
The Precision Medicine Initiative (PMI) is a national effort in the United States “to enable a new era of medicine through research, technology, and policies that empower patients, researchers, and providers to work together toward development of

Engineered metal nanoparticles in the sub-nanomolar levels kill cancer cells

April 18, 2016
Author(s)
Yasmine C. Daniels, William A. MacCrehan, Shinichiro Muramoto, Gheorghe NMN Stan, Vitaly Vodyanoy, Oleg Pustovyy
Zinc and copper metal nanoparticles were produced from bulk metal rods by an underwater high-voltage discharge method. The metal nanoparticles, with estimated diameters of 1 nm to 2 nm, were determined to be more than 85 % non-oxidized. Exposure of rat RG2

Combined Effects of High-Dose Bisphenol A and Oxidizing Agent (KBrO3) on Cellular Microenvironment, Gene Expression, and Chromatin Structure of Ku70-deficient Mouse Embryonic Fibroblasts

April 15, 2016
Author(s)
Erdem Coskun, Pawel Jaruga, Miral M. Dizdar, Natalie Gassman, Samuel Wilson
Background: Exposure to the environmental endocrine disrupting chemical bisphenol A (BPA) has been reported to alter global gene expression, induce epigenetic modifications, and interfere with the complex regulatory networks of cells. In addition to these

Inhibitors of DNA glycosylases as potential therapeutic drugs in cancer

April 4, 2016
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Nathan Donley, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Pawel Jaruga, Erdem Coskun, Amanda K. McCullough, R. S. Lloyd
Ionizing radiation and most chemotherapeutic agents kill tumor cells by damaging DNA. The efficacy of DNA-damaging agents may be influenced by increased DNA repair capacity in tumors that results from overexpression of DNA repair proteins. Inhibition of

Genetic circuit design automation

April 1, 2016
Author(s)
Alec Nielsen, Bryan Der, Jonghyeon Shin, Prashant Vaidyanathan, Douglas Densmore, Vanya M. Paralanov, Elizabeth Strychalski, David J. Ross, Christopher Voigt
Computation can be performed in living cells using DNA-encoded circuits that process sensory information and control biological functions. Their construction is time-intensive, requiring manual part assembly and balancing of regulator expression. We

Design and synthesis of a minimal bacterial genome

March 25, 2016
Author(s)
Elizabeth A. Strychalski, Clyde A. Hutchinson III, Ray-Yuan Chuang, Vladimir Noskov, Nacyra Assad-Garcia, Tom J. Deerinck, Mark H. Ellisman, John Gill, Krishna Kannan, Bogumil J. Karas, Li Ma, James F. Pelletier, Zhi-Qing Qi, Alexander Richter, Lijie Sun, Yo Suzuki, Billyana Tsvetanova, Kim S. Wise, Hamilton O. Smith, John I. Glass, Chuck Merryman, Daniel G. Gibson, J. C. Venter
We used whole-genome design and complete chemical synthesis to minimize the 1079–kilobase pair synthetic genome of Mycoplasma mycoides JCVI-syn1.0. An initial design, based on collective knowledge of molecular biology combined with limited transposon

SMALL MOLECULE INHIBITORS OF DNA GLYCOSYLASES AS POTENTIAL DRUGS IN CHEMO- AND RADIOTHERAPIES

March 19, 2016
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Nathan Donley, Marcus J. Calkins, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Amanda K. McCullough, R S. Lloyd, Erdem Coskun, Pawel Jaruga
Introduction: Ionizing radiation and most chemotherapeutic agents kill tumor cells by damag-ing DNA. The efficacy of DNA-damaging agents may be influenced by increased DNA repair capacity in tumors that results from overexpression of DNA repair proteins

Automation of Antimicrobial Activity Screening

March 12, 2016
Author(s)
Samuel P. Forry, Meggan C. Madonna, Daneli Lopez-Perez, Nancy J. Lin, Madeleine D. Pasco
ABSTRACT: Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were

Clinical Implications of Technical Performance in Medical Genome Sequencing

March 2, 2016
Author(s)
Justin M. Zook, James Priest, Rachel Goldfeder, Megan Grove, Daryl Waggott, Matthew Wheeler, Euan Ashley, Marc L. Salit
As next-generation sequencing is becoming routinely applied to clinical care, the predictive characteristics and limitations of whole exome and whole genome sequencing need to be well-understood. The Genome in a Bottle Consortium has recently published a

Method Validation and Standards in Hydrogen/Deuterium Exchange Mass Spectrometry

March 1, 2016
Author(s)
Jeffrey W. Hudgens, Richard Y. Huang, Emma D'Ambro
The chapter examines method validation and measurement standards for measuring protein dynamics by hydrogen/deuterium exchange mass spectrometry. Topics examined include the rationale for a reference measurement system for HDX-MS, attaining high accuracy

THE PROFICIENCY TEST (PILOT) REPORT OF THE GLOBAL MICROBIAL IDENTIFIER INITIATIVE YEAR 2014

March 1, 2016
Author(s)
Nathanael D. Olson, Rene S. Hendriksen, Susanne K. Pedersen, Mette V. Larsen, Jamie N. Pedersen, Oksana Lukjancenko, Rolf S. Kaas, Pimlapas Leekitcharoenphon, Lasse Bergmark, Inge M. Hansen, Vitali Sintchenko, William J. Wolfgang, Henrik T. Westh, Jacob Moran-Gilad, Isabel Cuesta, Angel Zaballos, Brian Beck, Anthony Underwood, Errol Strain, James Pettengill

Quantitative Flow Cytometry Measurements in Antibody Bound per Cell Based on CD4 Reference

February 12, 2016
Author(s)
Lili Wang, Heba Degheidy, Fatima Abbasi, Howard Mostowski, Gerald Marti, Steven R. Bauer, Robert Hoffman, Adolfas K. Gaigalas
Multicolor flow cytometer assays are with fluorescently labeled antibodies routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens either on the cell

A Description of the Clinical Proteomics Tumor Analysis Consortium (CPTAC) Common Data Analysis Pipeline

February 10, 2016
Author(s)
Jeri S. Roth, Paul A. Rudnick, Sanford Markey, Yuri Mirokhin, Xinjian Yan, Dmitrii Tchekhovskoi, Stephen Stein, Nathan J. Edwards, Ratna R. Thangudu, Karen A. Ketchum, Christopher R. Kinsinger, Mehdi Mesri, Henry Rodriguez
The Clinical Proteomic Tumor Analysis Consortium (CPTAC) has produced large proteomics datasets from the mass spectrometric interrogation of tumor samples previously studied by The Cancer Genome Atlas (TCGA) program. The availability of the genomic and

Dioctyl sodium sulfosuccinate (DOSS; CAS #577-11-7) a major component of the dispersant COREXIT is an obesogen

January 20, 2016
Author(s)
Alexis M. Temkin, Robert R. Bowers, Margaret E. Magaletta, Steven Holshouser, Adriana Maggi, Paolo Ciana, Louis J. Guillette Jr., John Bowden, John E. Baatz, Demetri D. Spyropoulos, John Kucklick
Background: Product safety testing typically involves lethal toxicity assessments, which likely miss long- term health impacts. Optimizing long-term product safety is critical for widespread chemical use. The obesity epidemic exemplifies a condition based

SVClassify: a method to use multiple datasets to classify candidate structural variants into true positives and false positives

January 16, 2016
Author(s)
Justin M. Zook, Hemang M. Parikh, Desu Chen, Hariharan K. Iyer, Marc L. Salit, Wolfgang Losert
The human genome contains variants ranging in size from small single nucleotide polymorphisms (SNPs) to large structural variants (SVs). While high-quality benchmark small variant calls have recently been developed by the Genome in a Bottle Consortium, no

ASTM Workshop on Standards & Measurements for Tissue Engineering Scaffolds

January 1, 2016
Author(s)
Carl G. Simon Jr., John Test, Michael Yaszemski, Reto Luginbuehl, Anthony Ratcliffe, Paul Tomlins
A “Workshop on Standards & Measurements for Tissue Engineering Scaffolds” was held on May 21, 2013 and was sponsored by the American Society of Testing and Materials International (ASTM International) in Indianapolis, IN. The purpose of the workshop was to
Displaying 451 - 475 of 1242
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