Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Automation of Antimicrobial Activity Screening

Published

Author(s)

Samuel P. Forry, Meggan C. Madonna, Daneli Lopez-Perez, Nancy J. Lin, Madeleine D. Pasco

Abstract

ABSTRACT: Manual and automated methods were compared for routine screening of compounds for antimicrobial activity. Automation generally accelerated assays and required less user intervention while producing comparable results. Automated protocols were validated for planktonic, biofilm, and agar cultures of the oral microbe Streptococcus mutans that is commonly associated with dental caries. Toxicity assays for the known antimicrobial compound cetylpyridinium chloride (CPC) were validated against planktonic, biofilm forming, and 24 h biofilm culture conditions, and several commonly reported toxicity/antimicrobial activity measures were evaluated: the 50% inhibition concentration (IC50), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Using automated methods, three halide salts of cetylpyridinium (CPC, CPB, CPI) were rapidly screened with no detectable effect on antimicrobial activity.
Citation
Journal of Microbial Biotechnology
Volume
6

Keywords

Antimicrobial activity, automation, biofilm, toxicity, microbial growth

Citation

Forry, S. , Madonna, M. , Lopez-Perez, D. , Lin, N. and Pasco, M. (2016), Automation of Antimicrobial Activity Screening, Journal of Microbial Biotechnology, [online], https://doi.org/10.1186/s13568-016-0191-2 (Accessed June 12, 2024)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created March 12, 2016, Updated November 10, 2018