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Health

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Displaying 726 - 750 of 848

Synchrotron White-Beam X-Ray Topography of Ribonuclease S Crystals

April 1, 2002
Author(s)
W M. Vetter, David Travis Gallagher, M Dudley
With careful experimental design indexed synchrotron white-beam X-ray topographs of ribonuclease S crystals at ambient temperature could be recorded with a definition and contrast comparable to that of monochromatic beam topographs of other proteins

DNA Hydrogel Plugs Immobilized in Microfluidic Channels with Improved Stability

February 28, 2002
Author(s)
Rebecca A. Zangmeister, K G. Olsen, David J. Ross, Michael J. Tarlov
It has been previously demonstrated that DNA, modified on the 5' end with an acrylic acid group, can be incorporated into a polyacrylamide hydrogel matrix. These types of DNA containing gels have recently been immobilized in plastic microfluidic channels

Development of Y STR Megaplex Assays

December 1, 2001
Author(s)
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Turkish Population Data With the CODIS Multiplex Short Tandem Repeat Loci

December 1, 2001
Author(s)
B S. Akbasak, B. Budowle, D J. Reeder, J Redman, Margaret C. Kline
Allele frequencies for thirteen tetrameric short tandem repeat (STR) loci, CSF1PO, D18S51, D3S1358, D21S11, D5S818, FGA, D7S820, HUMTH01, D8S1179, TPOX, D13S317, VWA, and D16S539 were determined on 198 Turkish blood samples.

Intron and Exons

October 17, 2001
Author(s)
Arlin B. Stoltzfus
An intron (or intervening sequence) is a segment of RNA excised from a gene transcript, with concomitant ligation of flanking segments called exons. Introns of some type are found in every kingdom of cellular life, and also in viruses, bacteriophages and

Temperature Measurement and Control in Microfluidic Systems

October 1, 2001
Author(s)
David J. Ross, Michael Gaitan, Laurie E. Locascio
We describe a technique for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. The efficiency of the method

Raman and FTIR Spectroscopies of Fluorescein in Solutions

August 1, 2001
Author(s)
Lili Wang, A. E. Roitberg, Curtis W. Meuse, Adolfas K. Gaigalas
Raman and FTIR transform-infra red (FT-IR) spectroscopies of fluorescein in aqueous solutions have been investigated in the pH range from 9.1 to 5.4. At pH 9.1 fluorescein is in the dianion form. At pH 5.4, fluorescein is a mixture of monoanion (

An MD/QM Study of the Chorismate Mutase-Catalyzed Claisen Rearrangement Reaction

July 1, 2001
Author(s)
S. E. Worthington, A. E. Roitberg, Morris Krauss
The reaction path for the rearrangement of chorismate to prephenate, catalyzed by chorismate mutase, has been calculated with ab initio quantum chemistry. The calculation of a reaction path is initiated from two catalytically competent conformations of the
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