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Displaying 751 - 775 of 852

Raman and FTIR Spectroscopies of Fluorescein in Solutions

August 1, 2001
Author(s)
Lili Wang, A. E. Roitberg, Curtis W. Meuse, Adolfas K. Gaigalas
Raman and FTIR transform-infra red (FT-IR) spectroscopies of fluorescein in aqueous solutions have been investigated in the pH range from 9.1 to 5.4. At pH 9.1 fluorescein is in the dianion form. At pH 5.4, fluorescein is a mixture of monoanion (

An MD/QM Study of the Chorismate Mutase-Catalyzed Claisen Rearrangement Reaction

July 1, 2001
Author(s)
S. E. Worthington, A. E. Roitberg, Morris Krauss
The reaction path for the rearrangement of chorismate to prephenate, catalyzed by chorismate mutase, has been calculated with ab initio quantum chemistry. The calculation of a reaction path is initiated from two catalytically competent conformations of the

The Claisen Rearrangement of an Unusual Substrate in Chorismate Mutase

July 1, 2001
Author(s)
S. E. Worthington, Morris Krauss
The calculated reaction path for an unusual substrate of chorismate mutase (Bacillus.subtilis) is found to be completely comparable to that of the native chorismate. In the unusual substrate, the cyclohexadienyl ring in chorismate is replaced by

Reference Materials for DNA Analysis

March 1, 2001
Author(s)
Barbara C. Levin, D J. Reeder
Mention has already been made, in Section 5.1. Of the use of DNA-based techniques for control of microbiological DNA. In parallel to the work of ATCC the USA National Institute of Standards and Technology (NIST) developed three Standard Reference Materials

The Development of Fluorescence Intensity Standards

March 1, 2001
Author(s)
Adolfas K. Gaigalas, L. Li, O. Henderson, R F. Vogt, J. Barr, G E. Marti, J. Weaver, A. Schwartz
The use of fluorescence as an analytical technique has been growing over the last 20 years. A major factor in inhibiting more rapid growth has been the inability to make comparable fluorescence intensity measurements across laboratories. NIST recognizes

Gene Expression Analysis on Medium-Density Oligonucleotide Arrays

January 1, 2001
Author(s)
R. Sinibaldi, C D. O'Connell, H Rodriguez, C. Seidel
As the human genome project continues toward its goal of sequencing the entire human genome by the end of 2003, this is providing unique opportunities for studying genetic variation in humans and its relationship with disease risk and aging. Consequently

Repair of oxidative DNA damage in Drosophila melanogaster: identification and characterization of dOgg1, a second DNA glycosylase activity for 8-hydroxyguanine and formamidopyrimidines

December 1, 2000
Author(s)
C. Dherin, M. Dizdaroglu, H. Doerflinger, S. Boiteux, J. P. Radicella
In Drosophila, the S3 ribosomal protein has been shown to act as a DNA glycosylase/AP lyase capable of releasing 8-hydroxyguanine (8-OH-Gua) in damaged DNA. Here we describe a second Drosophila protein (dOggl) with 8-OH-Gua and abasic (AP) site DNA repair
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