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We utilize scanning confocal fluorescence microscopy to interrogate single fluorescent lipid molecules in unsupported planar bilayers. At high photon flux, an optical trapping effect appears that is approximately 100,000 times larger than predicted by
John T. Woodward IV, Marlon L. Walker, Curtis W. Meuse, David J. Vanderah, G Poirier, Anne L. Plant
UV cleaned gold substrates incubated in solutions of alkanethiol show islands on the monolayer surface when imaged with non-contact atomic force microscopy (AFM). The height of the islands above the monolayer is approximately twice the height of the
L. Li, Curtis W. Meuse, Vitalii I. Silin, Adolfas Gaigalas, Y. Z. Zhang
Alexa 488 fluorophores was immobilized on a gold electrode. The formation of the layer of fluorophore was followed in real time using surface plasmon resonance (SPR). Both SPR and spectroscopic ellipsometry (SE) gave a thickness of about} 0.7 nm for the
Free radicals such as OH, e aq- and H atoms react with DNA by addition to bases or abstraction of H atoms from the sugar moiety. Oxygen adds to base or sugar radicals of DNA resulting in formation of peroxyl radicals. DNA radicals further react to give
The field of human mitochondrial genetics has advanced way beyond where the Human Genome Project hopes to be by the year 2003, the projected year for completing the sequence of the entire human nuclear DNA genome. Not only has the mitochondrial DNA (mtDNA)
T Petralli-Mallow, Kimberly A. Briggman, Lee J. Richter, John C. Stephenson, Anne L. Plant
Vibrational spectra of biomimetic membranes have been obtained using a broad-band approach to sum frequency generation (SFG). A new innovation, broad band SFG (BBSFG) allows for high quality SFG spectra with rapid collection times. With the BBSFG approach
Vytautas Reipa, S L. Yeh, H G. Monbouquette, V L. Vilker
Decanethiol was coadsorbed with tetradecylmethyl viologen (C 14MV; 1-Methyl-1 -tetradecyl-4,4 -bipyridinium chloride) on gold electrodes in order to improve stability of C 14MV as an electrochemical mediator for coupling to nitrate reductase enzymes
Margaret C. Kline, Janette W. Redman, David L. Duewer, D J. Reeder
The National Institute of Standards and Technology (NIST) has provided a number of educational interlaboratory exercises to the forensic community. The recent NIST Mixed Stain Study #2 consisted of three separate sets of materials. Set 1) three stains on
Polymers partitioning into highly confined spaces can be studied using single nanometer-scale pores formed by protein ion channels. The ionic conductance of a channel depends on the state of occupancy of the pore by polymer and serves as a measure of