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Displaying 701 - 725 of 848

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

Fluorescence Thermometry in Microfluidics

September 29, 2003
Author(s)
David J. Ross, Laurie E. Locascio
Two techniques are described for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. In the first technique, a single, strongly temperature-dependent fluorophore, rhodamine B, is used as the basis for

Choosing a Cantilever for In Situ Atomic Force Microscopy

April 1, 2003
Author(s)
John T. Woodward IV
This manuscript is a non-peer reviewed, invited tutorial for Microscopy Today. It describes the issues involved in choosing a cantilever for atomic force microscope (AFM) imaging under fluids. In situ AFM imaging is an increasingly popular technique for

Thin Films of Collagen Affect Smooth Muscle Cell Morphology

March 4, 2003
Author(s)
John T. Elliott, Alessandro Tona, John T. Woodward IV, P L. Jones, Anne L. Plant
The purpose of this study was to provide a reproducible method for applying collagen to surfaces on which cells can be grown, and to characterize the resulting thin films of collagen protein with respect to molecular structure and cellular response

Thermodynamics of Enzyme-Catalyzed Reactions

March 1, 2003
Author(s)
Robert N. Goldberg, Yadu D. Tewari
This review, which has been prepared for the McGraw-Hill 2003 Yearbook of Science and Technology, gives an overview of the thermodynamics of enzyme-catalyzed reactions. Topics include the thermodynamic formalism(s) used in this field, computational methods

Physics Laboratory: 2002 Activities, Accomplishments and Recognition

February 1, 2003
Author(s)
Jonathan E. Hardis, William R. Ott, G G. Wiersma
This report summarizes the research and measurement science carried out during calendar year 2001 in the NIST Physics Laboratory. The Laboratory supports U.S. industry, government, and the scientific community by providing measurement services and research

Design and Use of a Peptide Nucleic Acid for Detection of the Heteroplasmic Low-Frequency Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-Like Episodes (MELAS) Mutation in Human Mitochondrial DNA

December 1, 2002
Author(s)
Diane K. Hancock, Frederick P. Schwarz, F H. Song, L J. Wong, Barbara C. Levin
Most pathogenic human mitochondrial DNA (mtDNA) mutations are Heteroplasmic and are difficult to detect when present at very low levels in a population of normal mtDNA molecules. This paper describes a simple methodology to detect low levels of the single

Thermodynamic Quantities for the Ionization Reactions of Buffers in Water

December 1, 2002
Author(s)
Robert N. Goldberg, N Kishore, R M. Lennen
This table contains selected values for the pK, standard molar enthalpy of reaction {Δ} rH , and standard molar heat-capacity change {Δ} rC p for the ionization reactions of 64 buffers many of which are relevant to biochemistry and to biology. The values

A New NIST Mitochondrial DNA Interactive Website

August 1, 2002
Author(s)
Barbara C. Levin, L A. Tully, Diane K. Hancock, Frederick P. Schwarz, M S. Lee
Every human cell has from a few dozen to several thousand mitochondria each of which contain mitochondrial DNA (mtDNA). The sequence of the entire human mtDNA (16,569 base pairs) was determined and published by Anderson et al. in 1981. A mtDNA standard

Electrophoretic Capture of Circular DNA in Gels - A Review

August 1, 2002
Author(s)
B. Akerman, Kenneth D. Cole
Results on electrophoretic capture of circular DNA in porous gels are reviewed. Processes, which cause arrest of circular forms of DNA during electrophoresis, can provide a very efficient separation mechanism for the purification of plasmids and bacterial
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