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Temperature Measurement and Control in Microfluidic Systems



David J. Ross, Michael Gaitan, Laurie E. Locascio


We describe a technique for the measurement of fluid temperatures in microfluidic systems based on temperature-dependent fluorescence. The technique is easy to implement with a standard fluorescence microscope and CCD camera. The efficiency of the method is demonstrated by measuring temperature distributions resulting from Joule heating in a variety of microfluidic circuits that are electrokinetically pumped. Fluid temperatures ranging from room temperature to 70 degrees C were measured. Simultaneous spatial and temporal resolutions of 1 mm and 33 ms were achieved.
Micro Total Analysis Systems 2001
Publisher Info
Springer, dordrecht,


fluorescence, Joule heating, microfluidics, temperature


Ross, D. , Gaitan, M. and Locascio, L. (2001), Temperature Measurement and Control in Microfluidic Systems, Springer, dordrecht, (Accessed May 17, 2024)


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Created October 1, 2001, Updated February 19, 2017