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Displaying 351 - 375 of 848

Quantification of Cells with Specific Phenotypes I: Determination of CD4+ Cell Count per mL in Reconstituted Lyophilized Human PBMC Pre-labelled with Anti-CD4 FITC Antibody

March 10, 2015
Author(s)
Richard Stebbings, Lili Wang, Janet Sutherland, Martin Kammel, Adolfas Gaigalas, M. John, B. Roemer, M. Kuhne, R. J. Schneider, M. Braun, N. Leclere, D. Dikshit, Fatima Abbasi, Gerald Marti, P. Porcedda, M. Sassi, L. Revel, S. K. Kim, D. Marshall, L. Whitby, Jing Wang, V. Ost, M. Vonski, Joerg Neukammer
A surface-labelled lyophilised lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells (PBMC) pre-labelled with a fluorescein isothiocyanate (FITC) conjugated anti-CD4 monoclonal antibody. The sLL preparation is

Proceedings of SPIE

March 4, 2015
Author(s)
Maritoni A. Litorja, Aaron A. Urbas, Yuqin Zong
The fluorescent light detected by a clinical imager is assumed to be proportional to the amount of fluorescent substance present in the sample. Unfortunately, there are many factors that can add or subtract to the light signal directly attributable to the

Evaluation of Low-Contrast Detectability of Iterative Reconstruction across Multiple Institutions, CT Scanner Manufacturers, and Radiation Exposure Levels

February 19, 2015
Author(s)
Adele P. Peskin, Ganesh Saiprasad, James J. Filliben, Alden A. Dima
Purpose: To evaluate the impact of Iterative Reconstruction (IR) compared with Filtered Back Projection (FBP) using low-contrast objects in phantom images across vendors and exposure levels. Materials and Methods: Randomized repeat scans of the Gammex 464

Rapid DNA melting analyses using a microfabricated electrochemical platform

January 1, 2015
Author(s)
Zuliang Shen, Stephen Semancik, Herman O. Sintim
A new microscale platform is reported which is based on microfabrication methods that integrate thermal control and multi-electrode components to enable rapid temperature-dependent electrochemical measurements on small volume fluid samples. We demonstrate

Quantitative, label-free characterization of stem cell differentiation at the single-cell level by broadband coherent anti-Stokes Raman scattering microscopy

December 31, 2014
Author(s)
Marcus T. Cicerone, Young J. Lee, Khaled A. Aamer, Prabhas V. Moghe, Sebastian L. Vega, Parth J. Patel
We use broadband coherent anti-Stokes Raman scattering (BCARS) microscopy to characterize lineage commitment of individual human mesenchymal stem cells (hMSCs) cultured in adipogenic, osteogenic, and basal culture media. We treat hyperspectral images

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1992 Comparability Studies: Results for Round Robins XXIV, XXV, and XXVI Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 3 Ascorbic Acid in Human Serum

December 19, 2014
Author(s)
Jeanice M. Brown Thomas, Neal E. Craft, David L. Duewer, Kline Margaret, Sam A. Margolis, Willie E. May, Katherine E. Sharpless
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1993 Comparability Studies: Results for Round Robins XXVII, XXVIII, and XXIX Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 4 Ascorbic Acid in Human Se

December 19, 2014
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Kline Margaret, Sam A. Margolis, Willie E. May, Katherine E. Sharpless
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

December 11, 2014
Author(s)
Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D. Cole, Illarion Turko, Lili Wang, Fatima Abbasi
For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC)

Analysis of One-Minute Potentially Available Fluoride from Dentifrice

December 4, 2014
Author(s)
Clifton Carey, Erin Holahan
Previous reports found that some fluoride containing dentifrices do not release effective concentrations of fluoride during brushing. Failure to release fluoride can be due to dentifrice matrix components that interfere with the solubilization of the

High Resolution Surface Plasmon Resonance Imaging for Single Cells

November 30, 2014
Author(s)
Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However

Oxidatively Induced DNA Damage and Its Repair in Cancer

November 25, 2014
Author(s)
M Miral Dizdar
Oxidatively induced DNA damage is caused in living organisms by endogenous and exogenous reactive species. DNA lesions resulting from this type of damage are mutagenic and cytotoxic and, if not repaired, can cause genetic instability that may lead to

Genomic Reference Materials for Clinical Application

November 21, 2014
Author(s)
Justin M. Zook, Marc L. Salit
Reference Materials are well-characterized, homogeneous, and stable samples that can be used to understand measurement performance. The Genome in a Bottle Consortium is developing whole human genome DNA Reference Materials from large batches of DNA

Accurate determination of the size distribution for polydisperse, cationic metallic nanomaterials by asymmetric-flow field flow fractionation

November 13, 2014
Author(s)
Julien C. Gigault, Thao M. Nguyen, John M. Pettibone, Vincent A. Hackley
In this work we have developed and validated a methodology for determining the size distribution in polydisperse cationic nanoparticle (NP) samples using asymmetric-flow field flow fractionation (A4F), where known compositional influence of previously used

Archaeal DNA replication

November 3, 2014
Author(s)
Zvi Kelman, Lori M. Kelman
DNA replication is an essential process for all life forms. While the process is fundamentally conserved in the three domains of life, bioinformatics, biochemical, structural and genetic studies have demonstrated that the process and the proteins involved

Assessing technical performance in differential gene expression experiments with external spike- in RNA control ratio mixtures

September 25, 2014
Author(s)
Sarah A. Munro, Steven P. Lund, Patrick S. Pine, Hans Binder, Djork-Arne Clevert, Ana Conesa, Joaquin Dopazo, Mario Fasold, Sepp Hochreiter, Huixao Hong, Nederah Jafari, David P. Kreil, Pawel P. Labaj, Yang Liao, Simon Lin, Christopher E. Mason, Javier Santoyo, Steven J. Schrodi, Leming Shi, Wei Shi, Gordon K. Smyth, Nancy Stralis-Pavese, Zhenqiang Su, Charles Wang, Jian Wang, Joshua Xu, Zhan Ye, Yong Yang, Ying Yu, Paul Zumbo, Marc L. Salit
There is a critical need for standard approaches to assess, report and compare the technical performance of genome-scale differential gene expression experiments. Here we assess technical performance with a proposed standard ‘dashboard’ of metrics derived

An Average Enumeration Method of Hyperspectral Imaging Data for Quantitative Evaluation of Medical Device Surface Contamination

September 15, 2014
Author(s)
Hanh Le, Moon Kim, Jeeseong C. Hwang, Yi Yang, Pawneena U-Thainual, Jin Kang, Do-Hyun Kim
We propose a quantification method called Mapped Average Principal component analysis Score (MAPS) to enumerate the contamination coverage on common medical device surfaces. The method was adapted from conventional Principal Component Analysis (PCA) on non

Rbm24a and Rbm24b are required for normal somitogenesis

August 29, 2014
Author(s)
Samantha D. Maragh
We recently demonstrated that the gene encoding the RNA binding motif protein 24 (Rbm24)¬¬ is expressed during mouse cardiogenesis, and determined the developmental requirement for its zebrafish homologs Rbm24a and Rbm24b during cardiac development. We

Assuring Confidence in Measurements in Biomedical Research

August 28, 2014
Author(s)
Anne L. Plant, Laurie E. Locascio, Willie E. May, Patrick D. Gallagher
‘Irreproducibility’ is a symptom of a broad challenge in measurement that needs to be addressed. Establishing a robust measurement process that will lead to greater confidence in biomedical research results will require a concerted effort from experts.

Multiscale Correlative Measurements of Nanoparticles in Cells

August 26, 2014
Author(s)
Aric W. Sanders, Kavita M. Jeerage, Cindi Schwartz, Alexandra E. Curtin, Ann C. Chiaramonti Debay
Nanoparticles are emerging as invaluable tools in disease diagnosis, disease treatment and imaging contrast enhancement agents. The interactions of nanoparticles with host organisms are complex and affect biological systems over length scales that vary

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass

August 11, 2014
Author(s)
Michael W. Halter, Elianna Bier, Paul C. DeRose, Gregory A. Cooksey, Steven J. Choquette, Anne L. Plant, John T. Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist for
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