Shape-changing magnetic assemblies as high-sensitivity NMR-readable nanoprobes
Gary Zabow, Stephen Dodd, Alan Koretsky
Fluorescent and plasmonic probes have proven invaluable in the life sciences, but function poorly in optically inaccessible regions. Here we present radio-frequency addressable analogs that afford sensing opportunities similar to those of fluorescent sensors, but that are readable with standard NMR equipment. The sensors comprise magnetic nano-assemblies that reversibly reconfigure in response to chosen stimuli, yielding novel, geometry-dependent, dynamic NMR spectral signatures. Using such sensors, we demonstrate pH sensing, ionic strength mapping, cell metabolism tracking, and sensor multiplexing. The shape-changing sensors can be made from biocompatible materials, are detectable down to low femtomolar concentrations, and offer potential NMR spectral shifts a million-fold greater than those of traditional magnetic resonance spectroscopies. Inherent adaptability should allow the same sensing platform to measure numerous environmental and physiological indicators.
, Dodd, S.
and koretsky, A.
Shape-changing magnetic assemblies as high-sensitivity NMR-readable nanoprobes, Nature Nanotechnology, [online], https://doi.org/10.1038/nature14294
(Accessed September 30, 2022)