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Real time assessment of a new hydrolytically degradable and photo-clickable hydrogel for cartilage tissue engineering

Published

Author(s)

Alexander J. Neumann, Timothy P. Quinn, Stephanie J. Bryant

Abstract

Mw 20 000 8-arm PEG was functionalized with ε-Caprolactone (Sigma) and, subsequently, conjugated with 5-nobornene-2-carboxylic acid (Sigma). H NMR was used to confirm the final product. Bovine chondrocytes were isolated from full-depth articular cartilage harvested from the knee joints of skeletally immature calves through overnight digestion of cartilage pieces in Collagenase type II. After 1,2,3 and 4 weeks, samples were removed for analysis. Ultrasound measurements and determination of the compressive modulus were conducted using a custom-built bioreactor system. DNA content significantly increased between week 1 and week 2, suggesting an increase in total cell number (data not shown). Extensive cartilaginous matrix production was detected both qualitatively and quantitatively. Staining for collagen type II and aggrecan revealed increasing matrix deposition and matrix interconnectivity during the four weeks of culture. Interestingly, the total GAG content increased significantly from week 1 to week 2 and then remained constant. Total collagen content however, increased significantly over the course of four weeks. Ultrasonic measurements revealed a significant decrease of the signal between week 1 and week 2, increased again between week 2 and week 3 before it significantly decreased between week 3 and week 4. A-cellular degradation showed a decrease of initial modulus of app. 40% by week 2 and of app. 85% by week 4. Contrary, in the cellular constructs, the secant modulus increased from 6.45 kPA (day 0) to 10.3 kPA at week 2 and to 52.8 kPA at week 4.
Proceedings Title
Real time assessment of a new hydrolytically degradable and photo-clickable hydrogel for cartilage tissue engineering
Conference Dates
March 24-28, 2015
Conference Location
Las Vegas, NV, US
Conference Title
2015 Orthopaedic Research Society Annual Meeting

Keywords

Cartridge tissue engineering, degradable hydrogel, ultrasonic measurement

Citation

Neumann, A. , Quinn, T. and Bryant, S. (2015), Real time assessment of a new hydrolytically degradable and photo-clickable hydrogel for cartilage tissue engineering, Real time assessment of a new hydrolytically degradable and photo-clickable hydrogel for cartilage tissue engineering, Las Vegas, NV, US (Accessed June 21, 2024)

Issues

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Created March 27, 2015, Updated October 12, 2021