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Proceedings of SPIE



Maritoni A. Litorja, Aaron A. Urbas, Yuqin Zong


The fluorescent light detected by a clinical imager is assumed to be proportional to the amount of fluorescent substance present in the sample. Unfortunately, there are many factors that can add or subtract to the light signal directly attributable to the desired fluorescence emission, especially with fluorescence from inside the body imaged remotely. The quantification of fluorescence emission is feasible by calibrating the imager using the international system of units (SI)-traceable physical and material calibration artifacts such that the detector’s digital numbers (DN) can be converted to radiometric units. Here we focus on the effects of geometric factor as a contributor to the fluorescence measurement uncertainty.
Conference Dates
February 7-12, 2015
Conference Location
San Francisco, CA
Conference Title
Photonics West 2015


fluorescence imaging, radiometry, calibration, standard reference material for NIR, fluorometry


Litorja, M. , Urbas, A. and Zong, Y. (2015), Proceedings of SPIE, Photonics West 2015, San Francisco, CA (Accessed April 15, 2024)
Created March 4, 2015, Updated February 19, 2017