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Rapid DNA melting analyses using a microfabricated electrochemical platform

Published

Author(s)

Zuliang Shen, Stephen Semancik, Herman O. Sintim

Abstract

A new microscale platform is reported which is based on microfabrication methods that integrate thermal control and multi-electrode components to enable rapid temperature-dependent electrochemical measurements on small volume fluid samples. We demonstrate the device's capabilities by performing melting curve analyses for single-nucleotide polymorphism (SNP) detection.
Citation
Analytica Chimica ACTA
Volume
853

Keywords

DNA melting temperature: electrochemical melting, microfabrication, electrochemical platform, micro heater, single-nucleaotide polymorphism, methylene blue, square wave volammetry

Citation

Shen, Z. , Semancik, S. and Sintim, H. (2015), Rapid DNA melting analyses using a microfabricated electrochemical platform, Analytica Chimica ACTA, [online], https://doi.org/10.1016/j.aca.2014.10.025 (Accessed April 12, 2024)
Created December 31, 2014, Updated October 12, 2021