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Bioscience

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Displaying 876 - 900 of 1242

Standards in Gene Expression Microarray Experiments

August 1, 2006
Author(s)
Marc L. Salit
The use of standards in gene expression measurements with DNA microarrays is ubiquitous—they just are not yet the kind of standards that have yielded microarray gene expression profiles that can be readily compared across different studies and different

The Pseudo-mitochondrial Genome Influences Mistakes in Heteroplasmy Interpretation

July 21, 2006
Author(s)
Ryan L. Parr, Jennifer Maki, Brian Reguly, Gabriel D. Dakubo, A A. Aguirre, Roy Wittock, Kerry Robinson, J P. Jakupciak, Robert E. Thayer
Nuclear mitochondrial pseudogenes (numts) are a potential source of contamination during mitochondrial DNA PCR amplification. This paper is the first to fully sequence 29 paralogous nuclear DNA fragments that represent the entire mitochondrial genome. This

The evaluation of an autosomal SNP 12-plex assay

April 1, 2006
Author(s)
Peter M. Vallone, Amy E. Decker, Michael D. Coble, John M. Butler
SNPs have the potential to play a useful role in human identification testing. Small PCR amplicon sizes associated with SNP typing technologies make SNPs attractive for typing degraded DNA or other low copy number situations. SNP markers can be useful in

Analysis of DNA Single Nucleotide Polymorphisms by Mass Spectrometry

March 17, 2006
Author(s)
Peter Vallone, John Butler
Single nucleotide polymorphisms(SNP) are the most frequent form of DNA sequence variation in the human genome and are becoming increasingly useful as genetic markers for genome mapping studies, medical diagnostics, and human identity testing. The primer

Oxidative Stress and DNA Damage-DNA Repair System in Vascular Smooth Muscle Cells in Artery and Vein Grafts

March 1, 2006
Author(s)
S. H. McLaren, D. Gao, L. Chen, J. R. Eshleman, V. Dawson, M. A. Trush, V. Bohr, M. Dizdaroglu, G. M. Williams, C. Wei
Graft failure in coronary artery bypass grafts (CABGs) utilizing the saphenous vein is significantly higher than in those utilizing the internal mammary artery (IMA) or the radial artery (RA). While a number of studies have described this phenomenon

Repair of Formamidopyrimidines in DNA Involves Different Glycosylases - Role of the OGG1, NTH1, and NEIL1 Enzymes

December 9, 2005
Author(s)
J Hu, N de Souza-Pinto, K Haraguchi, Barbara A. Hogue, Pawel Jaruga, M M. Greenberg, Miral M. Dizdar, V. Bohr
2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA), are formed abundantly in DNA of cultured cells or tissues exposed to ionizing radiation or to other free radical-generating systems. We show here that FapyG
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