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Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes

Published

Author(s)

Dustin Levy, Kimberly A. Briggman

Abstract

The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of
Citation
Biophysical Journal
Volume
23

Keywords

cholesterol-lipid phase diagrams, hybrid bilayer membranes, SFG, supported bilayer membrane mimics, vibrational sum frequency
Created May 25, 2007, Updated January 27, 2020