Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes



Dustin Levy, Kimberly Briggman


The interactions between cholesterol and saturated phospholipids in hybrid bilayer membranes (HBMs) were investigated using the interface-sensitive technique of vibrational sum frequency spectroscopy (VSFS). The unique sensitivity of VSFS to order/disorder transitions of the lipid acyl chains was used to determine the main gel to liquid crystal phase transition temperature, Tm, for HBMs of binary cholesterol/phospholipid mixtures on octadecanethiolate self-assembled monolayers. The phase transition temperature and the breadth of the transition were shown to increase with cholesterol content, and the phase boundaries observed in the cholesterol/phospholipid HBMs were comparable to the published phase diagrams of binary cholesterol/phospholipid vesicles. A thermodynamic assessment of the cooperative units of theHBMphase transitions revealed the presence of<10nmdiameter domains that were independent of the cholesterol composition.
Biophysical Journal


cholesterol-lipid phase diagrams, hybrid bilayer membranes, SFG, supported bilayer membrane mimics, vibrational sum frequency


Levy, D. and Briggman, K. (2007), Cholesterol/Phospholipid Interactions in Hybrid Bilayer Membranes, Biophysical Journal, [online], (Accessed July 12, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created May 24, 2007, Updated October 12, 2021