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Bioscience

Projects/Programs

Keyword

Organizational Unit

Development of Reference Methods and Reference Materials for the Determination of Hormones in Human Serum

Ongoing

Steroid hormones and thyroid hormones serve an essential function by regulating gene transcription. Improving the accuracy of clinical assays for hormones will improve diagnoses and result in earlier treatments. Therefore, reference methods for the measurement of steroid and thyroid hormones have

Photograph of several clinical Standard Reference Materials showing labeled boxes, bottles, vials, ampoules and printed certificates.

Development of Reference Methods and Reference Materials for Clinical Diagnostic Markers

Ongoing

Reference measurement procedures and standard reference materials help ensure accuracy and comparability of routine clinical assays. In addition, this work allows U.S. clinical diagnostic manufacturers to comply with the European Union directive regarding in-vitro diagnostic medical devices (IVD MD)

Development of Reference Methods and Reference Materials for Proteins

Ongoing

Accuracy and comparability of clinical assays is essential for timely and accurate medical interventions. However, studies have shown that large variations in results can occur among routine clinical assays. A clinical comparison among 14 commercial assay platforms revealed more than 20-fold

Development of Reference Methods and Reference Materials for the Determination of Cardiac Troponin I in Human Serum

Ongoing

The clinical measurement of serum cardiac troponin I (cTnI) has become one of the most important tools in the diagnosis of acute myocardial infarction and myocardial damage. Unfortunately, considerable variability in clinical cTnI assay results is observed when comparing assays from different

NIST researcher pipetting solutions into 96-well plate for analysis

Development of Reference Methods and Reference Materials for Protein Biomarkers

Ongoing

Accuracy and reliability of clinical assays is essential for timely and accurate medical interventions. However, studies have shown that large variations in results can occur among routine clinical assays. In one example, a clinical comparison among 13 commercial cardiac troponin I assay platforms

Digital PCR

Ongoing

With appropriately validated assays, digital polymerase chain reaction (dPCR) determines the number of DNA targets (copies) per reaction partition, without the need for a standard curve. Reaction partitions can be in the form of fixed chambers in a microfluidic device, referred to as chamber digital

DNA Mixture Interpretation

Ongoing

Interlaboratory Studies Mixture interlaboratory studies provide a means to assess a laboratory's ability to process and interpret DNA mixtures. Initial ‘Mixed Stain’ studies (published in 2001) provided physical samples for DNA profiling with the first commercial STR typing tests. Later, mixture

Ecogenotoxicity Measurements of Nanoparticles

Completed

Intended Impact Understanding the potential environmental fate of NPs has direct implications to the US economy, as potential environmental and human health impacts of NPs is one of the main factors slowing the commercialization of this technology. Reducing the uncertainty regarding the

Electronic Biophysical Measurements

Ongoing

We develop measurements that leverage electronic signal transduction using FETs to maximize sensitivity and improve the resolution of biomolecular measurements. The techniques allow direct charge transduction during molecular interactions to quantify fundamental biophysical processes. Critically the

Map of the world with the engineering biology topics covered in Americas, Europe, and Asia meetings

Engineering Biology Metrics and Technical Standards for the Global Bioeconomy

Ongoing

Stakeholders from the Americas, Asia and Australia, and Europe and Africa, came together through a series of regional workshops in Washington D.C., Singapore, and Brussels, respectively, to identify specific areas for development, both technical and non-technical towards continued scale-up and economic growth across the bioeconomy.

Engineering Living Measurement Systems to Sense PFAS

Ongoing

Per- and polyfluoroalkyl substances, PFAS, are thermodynamically stable organic fluorinated chemicals that are resistant to traditional degradation pathways leading to concern for bioaccumulation. These compounds originate from several industrial manufacturing processes and are found in soil systems

Engineering of G Protein-Coupled Receptors

Ongoing

A trait of all life is the ability to sense and respond to changing environments, and the largest family of eukaryotic proteins that sense and respond to extracellular signals are called G Protein-Coupled Receptors (GPCRs). In humans, over 800 GPCRs detect a wide range of biological and chemical

ERCC 2.0: Developing a New Suite of RNA Controls

Completed

Requested RNA Control products include: Transcript isoforms; New and improved messenger RNA mimics and mixture formulations; microRNA and other small RNA molecules; and Cancer fusion transcripts. ERCC technical working groups are developing design specifications, RNA control products, and analysis

Evaluating Measurement Quality of Microfluidic Cell-based Assays

Completed

Impact and Customers Our work establishes metrics for measurement quality and reproducibility for cell-based assays within microfluidic devices. Many novel microfluidic devices are being created that permit new measurements or enable new biological functions to be tested; however, characterizations

Evaluating the Quality of Cell Counting Methods: Experimental Design and Statistical Analysis

Ongoing

Related Standards/Tools ISO 20391-2:2019 Biotechnology — Cell counting — Part 2: Experimental design and statistical analysis to quantify counting method performance COMET (Counting Method Evaluation Tool) A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391

Ammunition metal box with explosives sticker.

Explosives Decomposition Chemistry

Completed

Intended Impact Computational modeling and simulation will help in the invention of new explosives, the design of compounded mixtures of existing materials, and the engineering of delivered systems such as military ordnance. Objective Detailed chemistry is a necessary part of computational

Expression of Stable Isotopically Labeled Proteins for Use as Internal Standards for Mass Spectrometric Quantitation of Clinical Protein Biomarkers

Ongoing

Measurement of intact proteins by mass spectrometry can be problematic. In order to make proteins more amenable to mass spectrometric detection, the proteins are commonly digested with a proteolytic enzyme prior to analysis. Quantitation can then be performed by adding known amounts of isotopically

External RNA Controls Consortium

Completed

While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological activity, the results were irreconcilable and irreproducible. Industry leaders approached the National Institute of Standards and Technology (NIST) in 2003 for help with addressing

Extreme Atom Probe Tomography

Ongoing

Sub-nanometer-resolved 3-D chemical mapping of any atom in any solid continues to be an imperative goal of materials research. If reduced to practice, it would have profound scientific, engineering, and economic impacts on U.S. industries collectively worth hundreds of billions of dollars. Such

FAQs - Genome in a Bottle

Ongoing

Flow Cytometry Lab

Ongoing

Current Capabilities ERF value assignment measurement service for traceable ERF value assignment to microparticles, Aseptic sorting of cell lines, and primary cells Quantitative measurements of cell surface and intracellular biomarkers by estimating antibody bound per cell Accurate enumeration of

Fluid Suspensions and Emulsions

Ongoing

We aim to: Develop measurement capabilities to relate solution structure and viscosity through novel combinations of unique small volume, high shear rate rheometers with scattering and particle-imaging techniques. A major application of these capabilities is understanding self-association in fluids

Fluorescence Microscopy Benchmarking: Overview

Completed

A challenge in using microscopy is how to compare and validate data from different instruments, and how to determine that the microscope performance today is the same as yesterday. NIST is developing a practical, automated approach to implementing performance benchmarking methods that will

The inside of a DNA extraction robot hold tube in a rack

Forensic Genetics

Ongoing

Since the late 1980s, NIST has had scientists involved in DNA testing (aka "DNA Fingerprinting"). Early concerns over measurement accuracy and poor quality control of forensic DNA tests caused the Department of Justice to call upon NIST scientists to help with standards development and technology

Fundamental Interaction Mechanisms of Engineered Nanomaterials with DNA

Completed

Intended impact Nanotechnology research has resulted in the rapid creation of engineered nanomaterials with many foreseeable applications in medical imaging/diagnosis and in drug delivery. However, there is a notable scarcity of both acute and chronic human toxicity data for these new materials

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