Projects/Programs

Displaying 1 - 25 of 205

RM 8376 Microbial Pathogen DNA Standards for Detection and Identification

Ongoing

RM 8376 consists of 20 individual gDNA components from 19 bacterial strains and 1 human reference genome. Its purpose is to enable developers and regulators to evaluate the analytical performance of metagenomic next generation sequencing (mNGS) tools. The material is certified for chromosomal copy

AAPS Interlaboratory Study: Protein Aggregation and Immunogenicity

Ongoing

To ensure the safety and efficacy of protein-based biotherapeutics, manufacturers strive to avoid unwanted immune responses caused by the biotherapeutic. Protein immunogenicity is a complex topic and depends on multiple factors related to the product, patient profile, and treatment. 1 , 2

Advanced Data Exchange Standards for the Biomanufacturing Supply Chain

Ongoing

Objective The objective of this project is to develop measurement science that includes methods, workflow models, information meta-models, interoperability metrics, and prototype tools to help U.S. manufacturers reduce the time and cost associated with making supply chain data accessible for better

Advanced electron paramagnetic resonance spectroscopy for structural characterization of bionanomaterials for bioscience and biotechnology

Ongoing

EPR spectroscopic methods to measure distances in the nanometer range, collectively referred to as pulsed dipolar spectroscopies, have been applied to biomacromolecular systems containing a pair of spin labels (nitroxide spin labels) to determine tertiary/quaternary structures and to follow

Advanced Magnetic Imaging

Ongoing

Ultra-low field (ULF) MRI MRI systems are widely used for clinical diagnostics where imaging is typically done in high-field magnets ranging from 1.5 T to 7 T to achieve a manageable signal-to-noise ratio needed for short imaging times (few minutes) and high resolution (1 mm or less). Ultra-low

Advanced Materials Metrology

Ongoing

Electromagnetic devices cannot operate without the interaction of electromagnetic waves with materials, and the characterization of the interface between fields and materials will be a critical task for any device or metrology development from nanoscale to larger scales. Areas of impact over the

Advanced Spectral Analysis: Two-Dimensional Correlation Spectroscopy (2DCOS)

Ongoing

When a series of spectra are acquired from a sample as a function of temperature, pressure, electric field strength, concentration, or any experimental parameters, conventional spectral analysis is performed by monitoring the intensity change at a specific frequency. However, such a single frequency

Assay Development for Cell-Free Expression Systems

Ongoing

Current practices for characterizing cell-free expression systems are limited and unsuitable to advance these technologies beyond the state-of-the-art. To meet this measurement need, the Cellular Engineering Group is developing quantitative assays to benchmark cell extracts, reagents, and cell-free

Automated Live Cell Imaging for Quantification of Catalytic Rates in Individual Cells

Ongoing

In this study, the rates at which large numbers of individual living cells enzymatically degrade green fluorescent protein (GFP) was examined using automated fluorescence microscopy. A clonal population of NIH3T3 fibroblasts that resulted from stable transfection with a construct containing the

Bioelectronic Sensors for Tissue/Organ-on-a-Chip Systems

Ongoing

We apply our expertise in micro/nanofabrication, electrokinetics and cell-based assays to develop bioelectronic sensors in microfluidic platforms. We are working on a platform that entraps cells for electrochemical monitoring in an environment that has mechanical properties more similar to those

Biofabrication of Tissue Engineered Constructs

Ongoing

OCT imaging was assessed using a model scaffold-cell system consisting of a polysaccharide-based hydrogel seeded with human Jurkat cells. Four test systems were used: hydrogel seeded with live cells, hydrogel seeded with heat-shocked or fixed dead cells and hydrogel without any cells. Time series

Biofilm Models to Evaluate Structure-Function Relationships

Ongoing

Biofilms are complex 3D microbial structures that exist throughout nature including on our skin and gut mucosa, in waterways, and in soil. They are typically composed of multiple microbial species surrounded by proteins, polysaccharides, and extracellular DNA woven together into a hydrated matrix

Bioimaging

Ongoing

Imaging and image analysis are used ubiquitously in biological and biomedical research. One of the great advantages of imaging is its intuitive information content; much can be learned from just a glance at the data. On the other hand, these data generally contain a very large amount of untapped

Biomanufacturing Initiative

Ongoing

The objective of the NIST biomanufacturing program is to support US biopharmaceutical industry delivery of high quality and low cost protein drugs around the world. The mission is fulfilled through the development of standards, measurement science, and state of the art tools that support advances in

Biomaterials for Oral Health

Ongoing

NIST along with its partners has a long commitment to scientific excellence with demonstrated impact in improving the oral health of the public. NIST researchers are leaders in this field and create a community by engaging other researchers, industry, and regulatory agencies to address urgent needs

Bioprocessing Laboratory

Ongoing

Current Capabilities Computer controlled liquid nitrogen biostorage system Two culture areas including 4 monitored CO2 incubators 2 Six-foot BSLII biosafety cabinets Measurements- cell count and concentration, STR typing, flow cytometry, cell volume size distribution, conventional transmission and

Biosecurity for Synthetic Nucleic Acid Sequences

Ongoing

Emerging biotechnology, enabled by the ability to genetically engineer biological systems similar to the way we write software and program computers, can provide solutions to many pressing societal needs related to health, food security, supply chain resilience, and job growth for economic and

Biosurveillance and Pathogen Detection

Ongoing

NIST has established partnerships with other federal agencies to develop standards and measurement solutions to support biosurveillance. These efforts are supported in part through interagency agreements with partner agencies listed below to address their standards needs. PROGRAMS Standards for

Broadband Coherent Anti-Stokes Raman Scattering (BCARS) Microscopy

Ongoing

Impact There is a need for label-free chemical microscopy in medicine, biology and materials science. Most of the current methods use chemical labels that often disturb the distribution and nature of chemical components being investigated. The method we are developing enables noninvasive and rapid

Building Measurement Confidence for Cell Characterization

Ongoing

NIST collaborates with key stakeholders in industry, other government agencies, and academia to develop measurement assurance strategies and advanced measurement capabilities for cell characterization. Data and concepts developed through workshops and laboratory programs inform the development of

Cancer Biomarker Measurements and Collaborations

Ongoing

Collaborations and Cooperative Research Agreements We welcome collaborations and can help with assay validation and development of reference materials. Below are some examples of our collaborations and ways we can develop test materials. Early Detection Research Network (EDRN): Interagency agreement

Cancer Genome in a Bottle

Ongoing

Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cardiac Device Reliability

Ongoing

Impact and Customers This project will reduce the need for surgeries to replace failed implants, thereby improving safety and quality of life for implant recipients and reducing medical expense to society. It will provide assessment tools for the external electronic devices that support the

Cell Counting for Cell Therapies

Ongoing

Recent Publications and Resources for Cell Counting and Related Measurements Tools NOW AVAILABLE : COMET (Counting Method Evaluation Tool) , A Shiny Application that executes the statistical analysis and reporting outlined in ISO 20391-2:2019 https://cell-counting.shinyapps.io/COMET/ Standards ISO

Samples for Cell free DNA Methylation Measurements

Cell free DNA Methylation Measurements: Invitation to Participate in an Interlaboratory Testing Program Evaluating Methylated Cell free DNA Test Materials

Ongoing

For this study, one set of materials was prepared by LGC Clinical Diagnostics and contains non-methylated cfDNA based on whole genome amplification, and methylated cfDNA by in vitro methylation. Both duplicate the size range of cfDNA. The other set of materials was prepared at NIST and was made with

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