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Search Publications by: Frank Heinrich (Assoc)

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Displaying 1 - 25 of 36

Structural and Biophysical Properties of Farnesylated KRas Interacting with the Chaperone SmgGDS-558

October 4, 2022
Dennis J. Michalak, Bethany Unger, Ellen Lorimer, Alexander Grishaev, Carol Williams, Frank N. Heinrich, Mathias Losche
KRas is a small GTPase and membrane-bound signaling protein. Newly synthesized KRas is posttranslationally modified with a membrane-anchoring prenyl group. KRas chaperones are therapeutic targets in cancer due to their participation in trafficking oncogeni

Copper-binding anticancer peptides from the piscidin family: an expanded mechanism that encompasses physical and chemical bilayer disruption

June 16, 2021
Fatih Comert, Frank N. Heinrich, Ananda Chowdhury, Mason Schoeneck, Caitlin Darling, Kyle Anderson, M. Daben J. Libardo, Alfredo Angeles-Boza, Vitalii I. Silin, Myriam Cotten, Mihaela Mihailescu
In the search for novel broad‑spectrum therapeutics to fight chronic infections, inflammation, and cancer, host defense peptides (HDPs) have garnered increasing interest. Characterizing their biologically‑active conformations and minimum motifs for

Spatial Distribution of PEO-PPO-PEO Block copolymer and PEO Homopolymer in Lipid Bilayers

April 7, 2020
Mihee Kim, Frank Heinrich, Greg Haugstad, Guichuan Yu, Guangcui Yuan, Sushil K. Satija, Wenjia Zhang, Hannah S. Seo, Joseph M. Metzger, Samira M. Azarin, Timothy P Lodge, Benjamin J. Hackel, Frank S. Bates
Maintaining the integrity of cell membranes is indispensable for cellular viability. Poloxamer 188 (P188), a poly(ethylene oxide)-b-poly(propylene oxide)-b-poly(ethylene oxide) (PEO-PPO-PEO) triblock copolymer with a number average molecular weight of 8

Structural Investigations of Membrane-Associated Proteins by Neutron Reflectometry

July 1, 2019
Rebecca Eells, David Hoogerheide, Paul A. Kienzle, Mathias Loesche, Charles Majkrzak, Frank Heinrich
Neutron reflectometry is a powerful technique for probing the structure of lipid bilayer membranes and membrane-associated proteins. Measurements of the specular neutron reflectivity as a function of momentum transfer can be performed in aqueous

Optimization of Reflectometry Experiments using Information Theory

February 1, 2019
Bradley W. Treece, Paul A. Kienzle, David Hoogerheide, Charles Majkrzak, Mathias Loesche, Frank Heinrich
A method based on Bayesian statistics and information theory is developed to analyze the information gain Δ}H of surface-sensitive reflectometry experiments. After presenting the underlying mathematical framework and its implementation, the method is

Neutron Scattering in the Biological Sciences: Progress and Prospects

December 1, 2018
Rana Ashkar, Hassina Z. Bilheux, Heliosa Bordallo, Robert Briber, David J. E. Callaway, Xiaolin Cheng, Xiang-Qiang Chu, Joseph E. Curtis, Mark Dadmun, Paul Fenimore, David Fushman, Frank Gabel, Kushol Gupta, Frederick Herberle, Frank Heinrich, Liang Hong, John Katsaras, Zvi Kelman, Eugenia Kharlampieva, Gerald R. Kneller, Andrey Kovalevsky, Susan T. Krueger, Paul Langan, Raquel Lieberman, Yun Liu, Mathias Losche, Edward Lyman, Yimin NMN Mao, John Marino, Carla Mattos, Flora Meilleur, Peter Moody, Jonathan D. Nickels, William B. O'Dell, Hugh O'Neill, Ursula Perez-Salas, Judith Peters, Loukas Petridis, Alexei P. Sokolov, Robert Sutherland, Norman Wagner, Michael NMN Weinrich, Kevin Weiss, Troy Wymore, Yang Zhang, Jeremy C. Smith
The scattering of neutrons can be used to provide information on the structure and dynamics of biological systems on multiple scales of length and time. Pursuant to an NSF-funded workshop in February 2018, we review recent developments in this field as

Insertion of Dengue E into Lipid Bilayers Studied by Neutron Reflectivity Molecular Dynamics Simulations

February 13, 2018
Juan M. Vanegas, Frank Heinrich, David M. Rogers, Bryan D. Carson, Sadie La Bauve, Briana C. Vernon, Sushil K. Satija, Aihua Zheng, Margaret Kielian, Susan B. Rempe, Michael S. Kent
The envelope (E) protein of Dengue virus rearranges to a trimeric hairpin to mediate fusion of the viral and target membranes, which essential for infectivity. Insertion of E into the target membrane serves to anchor E and possibly also to disrupt local

Protein Adsorption and Layer Formation at the Stainless Steel - Solution Interface Mediates Shear-Induced Particle Formation for an IgG1 Monoclonal Antibody

February 9, 2018
Cavan K. Kalonia, Frank Heinrich, Joseph E. Curtis, Sid Raman, Maria A. Miller, Steven Hudson
Passage of specific protein solutions through certain pumps, tubing, and/or filling nozzles can result in the production of unwanted subvisible protein particles (SVP). In this work, surface mediated SVP formation was investigated. Specifically, the

The Cytosolic Domain of T Cell Receptor z Associates with Membranes in a Dynamic Equilibrium and and Deeply Penetrates the Bilayer

September 11, 2017
Kerstin Zimmermann, Rebecca Eells, Frank Heinrich, Stefanie Rintoul, Brian Patrick Josey, Prabhanshu Shekhar, Mathias Loesche, Lawrence J. Stern
Interactions between lipid bilayers and the membrane-proximal regions of membrane-associated proteins play important roles in regulating membrane protein structure and function. The T cell antigen receptor (TCR) is an assembly of eight single-pass membrane

Fast Formation of Low-Defect-Density Tethered Bilayers by Fusion of Multilamellar Vesicles

January 10, 2017
Tadas Ragaliauskas, Mindaugas Mickevicius, Bozena Rakovska, Tadas Penkauskas, Frank Heinrich, Gintaras Valincius
Multilamelar vesicles fuse to molecular-anchor-grafted surfaces yielding low defect density, tethered bilayer membranes. Continuous bilayers are formed within 10 min, while the electrically insulating bilayers with less than 0,1 υmu-1} defect density can

Segmental Deuteration of a-Synuclein for Neutron Reflectometry on Tethered Bilayers

January 5, 2017
Zhiping Jiang, Frank Heinrich, Ryan P. McGlinchey, James M. Gruschus, Jennifer C. Lee
Neutron reflectometry (NR) is uniquely suited for studying protein interaction with phospholipid bilayers. While molecular details along the bilayer normal can be obtained on an Angstrom scale, NR cannot discern specific membrane-bound protein regions due

HIV-1 Matrix-31 Membrane Binding Peptide Interacts Differently with Membranes Containing PS vs. PI(4,5)P 2

September 15, 2016
Lauren O'Neil, Kathryn Andenoro, Isabella Pagano, Laura Carroll, Leah Langer, Zachary Dell, Davina Perera, Bradley W. Treece, Frank Heinrich, Mathias Loesche, John F. Nagle, Stephanie Tristram-Nagle
Efficient assembly of HIV-1 at the plasma membrane (PM) of the T-cell specifically requires PI(4,5)P 2. It was previously shown that a highly basic region (HBR) of the matric protein (MA) on the Gag precursor polyprotein Pr55^gag^ is required membrane

Membrane Binding of HIV-1 Matrix Protein: Dependence on Bilayer Composition and Protein Lipidation

May 1, 2016
Marilia Barros, Frank Heinrich, Siddhartha A. K. Datta, Alan Rein, Ioannis Karageorgos, Hirsh Nanda, Mathias Loesche
Retroviral Gag polyprotein is the structural determinant that assembles in a protein lattice on the host's plasma membrane to trigger formation of the viral protein/membrane shell. In this process, it employs multiple signals - electrostatic, hydrophobic

A Bacteriophage Endolysin that Eliminates Intracellular Streptococci

March 15, 2016
Yang Shen, Marilia Barros, Tarek Vennemann, David Travis Gallagher, Yizhou Yin, Sara B. Linden, Ryan D. Heselpoth, Dennis J. Spencer, David M. Donovan, John Moult, Vincent A. Fischetti, Frank Heinrich, Mathias Loesche, Daniel C. Nelson
PlyC, a bacteriophage-encoded A-B endolysin, lyses Streptococcus pyogenes (Spy) on contact and protects mice from upper respiratory Spy colonization. Here, we demonstrate that PlyC is a novel, potent agent for targeting and controlling intracellular Spy

The PTEN Tumor Suppressor Forms Homodimers in Solution

August 21, 2015
Frank Heinrich, Srinivas Chakravarthy, Hirsh Nanda, Antonella Papa, Pier Paolo Pandolfi, Alonzo H. Ross, Rakesh K. Harishchandra, Arne Gericke, Peter M. Loesche
The PTEN tumor suppressor exerts phosphatase activity on PI(3,4,5)P 3 in the plasma membrane and was recently reported to dimerize in cell models. Here we show that PTEN forms homodimers in vitro and determine a structural model of the complex from SAXS

Deuteration in Biological Neutron Reflectometry

June 30, 2015
Frank N. Heinrich
Neutron reflectometry is uniquely positioned in structural biology, because of its ability to characterize biomimetic interfacial architectures like lipid membranes and membrane-associated proteins non-destructively and in their native environment

Structural Features of Membrane-bound Glucocerebrosidase and a-Synuclein Probed by Neutron Reflectometry and Fluorescence Spectroscopy

January 9, 2015
Thai Leong Yap, Zhiping Jiang, Frank Heinrich, James M. Gruschus, Candace M. Pfefferkorn, Marilia Barros, Joseph E. Curtis, Ellen Sidransky, Jennifer C. Lee
Mutations in glucocerebrosidase (GCase), the enzyme deficient in Gaucher disease, are a common genetic risk factor for the development of Parkinson disease (PD) and related disorders, implicating the role of this lysosomal hydrolase in the disease etiology

Structure and Stability of Phospholipid Bilayers Hydrated by a Room-Temperature Ionic Liquid/Water Solution: a Neutron Reflectometry Study

October 23, 2014
Antonio Benedetto, Frank Heinrich, Miguel A. Gonzalez, Giovanna Fragneto, Erik Watkins, Pietro Ballone
Neutron reflectometry measurements (NR) have been carried out to probe the structure and stability of two model bio-membranes consisting of POPC and DMPC phospholipid bilayers hydrated by water solutions of two prototypical room-temperature ionic liquids