Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

HIV-1 Matrix-31 Membrane Binding Peptide Interacts Differently with Membranes Containing PS vs. PI(4,5)P2



Lauren O'Neil, Kathryn Andenoro, Isabella Pagano, Laura Carroll, Leah Langer, Zachary Dell, Davina Perera, Bradley W. Treece, Frank Heinrich, Mathias Loesche, John F. Nagle, Stephanie Tristram-Nagle


Efficient assembly of HIV-1 at the plasma membrane (PM) of the T-cell specifically requires PI(4,5)P2. It was previously shown that a highly basic region (HBR) of the matric protein (MA) on the Gag precursor polyprotein Pr55^gag^ is required membrane association. MA is N-terminally myristoylated, which enhances its affinity to membranes. In this work we used X-ray scattering and neutron reflectivity to determine how the physical properties and structure of lipid bilayers respond to the addition of binding domain peptides, either in the myristoylated form (MA31myr) or without the myristoyl group (MA31). Neutron reflectivity measurements showed the peptides predominantly located in the hydrocarbon interior. Diffuse X-ray scattering showed differences in membrane properties upon addition of peptides and the direction of the changes depended on lipid composition. As softening the host cell's PM upon contact with the protein lowers the free energy for membrane restructuring, thereby potentially facilitating budding of viral particles, or results suggest that the role of PI(4,5)P2 in viral assembly goes beyond specific stereochemical membrane binding. These studies reinforce the importance of lipids in virology.
Biochimica Et Biophysica Acta-Biomembranes


HIV Gag MA, neutron reflectometry, x-ray scattering, bending modulus


O'Neil, L. , Andenoro, K. , Pagano, I. , Carroll, L. , Langer, L. , Dell, Z. , Perera, D. , Treece, B. , Heinrich, F. , Loesche, M. , Nagle, J. and Tristram-Nagle, S. (2016), HIV-1 Matrix-31 Membrane Binding Peptide Interacts Differently with Membranes Containing PS vs. PI(4,5)P<sub>2</sub>, Biochimica Et Biophysica Acta-Biomembranes, [online], (Accessed June 23, 2024)


If you have any questions about this publication or are having problems accessing it, please contact

Created September 14, 2016, Updated October 12, 2021