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HIV-1 Matrix-31 Membrane Binding Peptide Interacts Differently with Membranes Containing PS vs. PI(4,5)P2

Published

Author(s)

Lauren O'Neil, Kathryn Andenoro, Isabella Pagano, Laura Carroll, Leah Langer, Zachary Dell, Davina Perera, Bradley W. Treece, Frank Heinrich, Mathias Loesche, John F. Nagle, Stephanie Tristram-Nagle

Abstract

Efficient assembly of HIV-1 at the plasma membrane (PM) of the T-cell specifically requires PI(4,5)P2. It was previously shown that a highly basic region (HBR) of the matric protein (MA) on the Gag precursor polyprotein Pr55^gag^ is required membrane association. MA is N-terminally myristoylated, which enhances its affinity to membranes. In this work we used X-ray scattering and neutron reflectivity to determine how the physical properties and structure of lipid bilayers respond to the addition of binding domain peptides, either in the myristoylated form (MA31myr) or without the myristoyl group (MA31). Neutron reflectivity measurements showed the peptides predominantly located in the hydrocarbon interior. Diffuse X-ray scattering showed differences in membrane properties upon addition of peptides and the direction of the changes depended on lipid composition. As softening the host cell's PM upon contact with the protein lowers the free energy for membrane restructuring, thereby potentially facilitating budding of viral particles, or results suggest that the role of PI(4,5)P2 in viral assembly goes beyond specific stereochemical membrane binding. These studies reinforce the importance of lipids in virology.
Citation
Biochimica Et Biophysica Acta-Biomembranes
Volume
1858

Keywords

HIV Gag MA, neutron reflectometry, x-ray scattering, bending modulus

Citation

O'Neil, L. , Andenoro, K. , Pagano, I. , Carroll, L. , Langer, L. , Dell, Z. , Perera, D. , Treece, B. , Heinrich, F. , Loesche, M. , Nagle, J. and Tristram-Nagle, S. (2016), HIV-1 Matrix-31 Membrane Binding Peptide Interacts Differently with Membranes Containing PS vs. PI(4,5)P<sub>2</sub>, Biochimica Et Biophysica Acta-Biomembranes, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=920960 (Accessed November 30, 2023)
Created September 14, 2016, Updated October 12, 2021