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Search Publications by: Sheng Lin-Gibson (Fed)

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Displaying 1 - 25 of 115

Report from the 2022 NIST Rapid Microbial Testing Methods (RMTM) Workshop

August 15, 2023
Stephanie Servetas, Nancy Lin, Nadratun Chowdhury, Scott Jackson, Jason Kralj, Sheng Lin-Gibson, Melody Sanders, Tara Eskandari
Safety and quality of advanced therapies, including cellular, gene, and tissue-engineered medical products, is paramount for success of these products. Sterility assurance testing confirming the absence of microbial contamination in advanced therapy

Report from the NIST Workshop: Launch of the Rapid Microbial Testing Methods Consortium

September 27, 2022
Nancy Lin, Scott Jackson, Stephanie Servetas, Kirsten Parratt, Joy Dunkers, Tara Eskandari, Sheng Lin-Gibson
On September 17, 2020, NIST hosted a virtual workshop to launch the Rapid Microbial Testing Methods (RMTM) Consortium. The RMTM Consortium aims to address the need for measurements and standards to increase confidence in the use of rapid testing for

MALDI TOF MS Analysis of Chan End Functonalized Polymers

October 12, 2021
Kathleen M. Flynn, Barry J. Bauer, Kathryn Beers, S Bencherif, H M. Byrd, Sheng Lin-Gibson
Recently, we and other research laboratories have developed methods whereby an organic species is covalently attached to the polymer forming an organic salt to analyze by matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI

Reference standards for accurate validation and optimization of assays that determine integrated Lentiviral vector copy number in transduced cells

July 11, 2021
Barbara S. Paugh, Lajos Baranyi, Andre Roy, Hua-Jun He, Lindsay Harris, Kenneth D. Cole, Caroline Raimund, Patricia S. Langan, Moria Artlip, Srikanta Jana, Rimas J. Orentas, Sheng Lin-Gibson, Winfried Krueger, Boro Dropuli?
Lentiviral vectors (LV) have emerged as a robust technology for therapeutic gene delivery into human cells as advanced medicinal products. As these products are increasingly commercialized, there are concomitant demands for their characterization to ensure

Recent Industrial Roadmaps to Enable Smart Manufacturing of Biopharmaceuticals

January 29, 2021
Sheng Lin-Gibson, Vijay Srinivasan
Biopharmaceutical manufacturing is an evolving industry with great potential to improve public health, but laden with technical and operational challenges. It has many features that are similar to other smart manufacturing industries, but it also has some

Methods for Characterization of Bioactivity Using Confocal Microscopy

December 1, 2020
Jirun Sun, Nancy Lin, Joy Dunkers, Sheng Lin-Gibson
One common tissue engineering approach for regenerating or replacing damaged tissues involves a porous polymeric scaffold. The scaffolds serve as the mechanical framework for cell attachment and growth, and generate an environment with features that span

Workshop 13: Building Measurement Assurance in Flow Cytometry

June 17, 2019
Lili Wang, Stephen Perfetto, Robert Hoffman, John T. Elliott, Sheng Lin-Gibson, Steven Bauer, Heba Degheidy, Judith Arcidiacono, Litwin Virginia
Two workshops were held to identify measurement challenges and potential solutions for building measurement assurance for flow cytometry. This report summarizes key findings, including the need for high quality reagents, reference standards or materials

Experimental and statistical methods to evaluate antibacterial activity of a quaternary pyridinium salt on planktonic, biofilm-forming, and biofilm states

March 3, 2017
Daneli Lopez-Perez, Paula J. Baker, Adam L. Pintar, Jirun Sun, Nancy Lin, Sheng Lin-Gibson
Robust evaluation and comparison of antimicrobial technologies are critical to improving biofilm prevention and treatment. Herein, a multi-pronged experimental framework and statistical models were applied to determine the effects of quaternary pyridinium

Relationship Between Quantitative Dispersion and Physical Properties of PMMA/SWNT Nanocomposites

February 19, 2017
Takashi Kashiwagi, Jeffrey Fagan, Keith Yamamoto, A N. Heckert, Stefan D. Leigh, Jan Obrzut, F Du, Sheng Lin-Gibson, Minfang Mu, K Winey, R Haggenmueller, Jack F. Douglas
Six PMMA/SWNT(0.5 wt%) nanocomposite samples intended to have different dispersion levels were prepared by the coagulation method using six different concentrations of SWNTs in DMF from 0.05 mg/ml to 1.2 mg/ml. The morphology of the samples was determined

Strategies for Achieving Measurement Assurance for Cell Therapy Products

June 1, 2016
Carl G. Simon Jr., Anne L. Plant, Sumona Sarkar, John T. Elliott, Sheng Lin-Gibson
Cell therapy products (CTPs) need quantitative, validated, and robust assays to support informed decision making during their development, manufacturing, and regulation. These products, the materials involved in their production and the methods for

Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

April 19, 2014
Subhadip Bodhak, Sheng Lin-Gibson, Carl G. Simon Jr., Luis Fernandez de Castro Diaz, Pamela G. Robey, Sergei A. Kuznetsov, Tina Kilts, Marian F. Young
The use of in vivo transplantation assays under defined experimental conditions has become a valuable standard for evaluating osteogenic potential of different stem cell populations and is the subject of ongoing pre-clinical reconstructive therapy research

Effects of nanoparticle size and charge on interactions with self-assembled collagen

March 1, 2014
Dongbo Wang, Jing (. Ye, Steven Hudson, Keana Scott, Sheng Lin-Gibson
Recent insights into bone formation have suggested that the critical first step to the biomineralization process is the integration of small (nanometer dimension) mineral clusters into collagen fibers. Such behavior is of intrinsic interest for the areas

Airbrushed Nanofiber Scaffolds Support Bone Marrow Stromal Cell Differentiation

November 1, 2013
Carl G. Simon Jr., Wojtek J. Tutak, Sumona Sarkar, Tanya M. Farooque, Jyotsnendu J. Giri, Dongbo Wang, Sheng Lin-Gibson, Joachim Kohn, Durgadas Bolikal
Nanofiber scaffolds are effective for tissue engineering since they emulate the fibrous nanostructure of native extracellular matrix (ECM). Although electrospinning has been the most common approach for fabricating nanofiber scaffolds, airbrushing

Effects of dual antibacterial agents MDPB and nano-silver in primer on microcosm biofilm, cytotoxicity and dentin bond properties

February 1, 2013
Ke Zhang, Lei Cheng, Satoshi Imazato, Joseph M. Antonucci, Nancy Lin, Sheng Lin-Gibson, Yuxing Bai, Hockin H. Xu
Objectives: Secondary caries at restoration margins is the main reason for restoration failure. Antibacterial bonding agents are promising to inhibit biofilm acids at the tooth-restoration interfaces. The objective of this study was to investigate the

Effect of Polymer Degree of Conversion on Streptococcus mutans Biofilms

October 5, 2012
Alison M. Kraigsley, Kathy Tang, John A. Howarter, Katrice Lippa, Sheng Lin-Gibson, Nancy Lin
The effects of key material properties of polymeric composite restorations on cariogenic oral biofilms are needed to provide insight into the materials' role in recurrent caries. The objective of this study was to quantify the effects of degree of