Killing bacteria impregnated in human dentin blocks via primer containing dimethylaminododecyl methacrylate
Chen Chen, Cheng Lei, Michael D. Weir, Nancy Lin, Sheng Lin-Gibson, Xuedong Zhou
Recent studies developed antibacterial bonding agents and composites containing dimethylaminododecyl methacrylate (DMADDM). The objectives of this study were to: (1) explore the effect of antibacterial primers containing DMADDM on inhibiting the Streptococcus mutans (S. mutans) impregnated into dentin blocks for the first time, and (2) compare the antibacterial efficacy of DMADDM with a previous quaternary ammonium dimethacrylate (QADM). Scotchbond Multi-Purpose (SBMP) bonding agent was used. DMADDM and QADM were mixed into SBMP primer. The following six primers were tested: SBMP control primer P, P + 2.5% DMADDM, P + 5% DMADDM, P + 7.5% DMADDM, P + 10% DMADDM, and P + 10% QADM. S. mutans were impregnated into dentin blocks. Then each primer was applied to the dentin block to test the killing of bacteria in dentinal tubules. Bacteria in dentin blocks were collected by a sonication method, and the viable colony-forming units (CFU) were measured. The bacteria inhibition zone of P + 10% DMADDM was ten times that of control primer (p < 0.05). CFU in dentin with P + 10% DMADDM was reduced by three orders of magnitude, compared to control group. DMADDM had much stronger antibacterial effect than QADM, and the efficacy increased with increasing DMADDM concentration. Dentin shear bond strengths were similar among all groups (p > 0.1). In conclusion, antibacterial primer with the new DMADDM was validated to kill bacteria inside dentin blocks, possessing a much stronger antibacterial potency than a previous QADM. Bonding agent containing DMADDM was effective in eradicating bacteria in dentin, and its efficacy was directly proportional to DMADDM mass fraction. DMADDM may be promising for use in bonding agents as well as in other restoratiove and preventive materials.
, Lei, C.
, Weir, M.
, Lin, N.
, Lin-Gibson, S.
and Zhou, X.
Killing bacteria impregnated in human dentin blocks via primer containing dimethylaminododecyl methacrylate, International Journal of Oral Science
(Accessed May 29, 2023)