NOTICE: Due to a lapse in annual appropriations, most of this website is not being updated. Learn more.

Form submissions will still be accepted but will not receive responses at this time. Sections of this site for programs using non-appropriated funds (such as NVLAP) or those that are excepted from the shutdown (such as CHIPS and NVD) will continue to be updated.

U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

PUBLICATIONS

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

Published

Author(s)

Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang

Abstract

Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 µg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies.
Citation
International Journal of Molecular Sciences
Pub Type
Journals

Download Paper

Local Download
Health, Flow cytometry, Clinical diagnostics, Cell measurements and Bioscience

Citation

Tian, L. , Elsheikh, E. , Patrone, P. , Kearsley, A. , Gaigalas, A. , Inwood, S. , Lin-Gibson, S. , Esposito, D. and Wang, L. (2021), Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19, International Journal of Molecular Sciences, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=931659 (Accessed October 6, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact [email protected].

Created March 8, 2021, Updated September 29, 2025
Was this page helpful?