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Displaying 101 - 125 of 1166

The science of the host-virus network

November 24, 2021
Author(s)
Gregory Albery, Daniel J. Becker, Liam Brierley, Cara Brook, Rebecca Christofferson, Lily Cohen, Tad Dallas, Evan Eskew, Anna Fagre, Maxwell Farrell, Emma Glennon, Sarah Guth, Maxwell Joseph, Nardus Mollentze, Ben Neely, Timothee Poisot, Angela Rasmussen, Sadie Ryan, Anna Siodin, Stephanie Seifert, Erin Sorrell, Colin Carlson
Better methods to predict and prevent the emergence of zoonotic viruses could support future efforts to reduce the risk of epidemics. We propose a network science framework for understanding and predicting human and animal susceptibility to viral

Liver proteome response to torpor in a basoendothermic mammal, Tenrec ecaudatus, provides insights into the evolution of homeothermy

October 6, 2021
Author(s)
Jane Khudyakov, Michael Treat, Mikayla Shanafelt, Jared Deyarmin, Ben Neely, Frank van Breukelen
Many mammals use adaptive heterothermy (e.g., torpor, hibernation) to reduce metabolic demands of maintaining high body temperature (Tb). Torpor is typically characterized by coordinated declines in Tb and metabolic rate (MR) followed by active rewarming

Characteristics to Consider when Selecting a Positive Control Material for an In Vitro Assay

September 29, 2021
Author(s)
Elijah Petersen, Andrew Nguyen, Jeffrey Brown, John T. Elliott, Amy Clippinger, John Gordon, Nicole Kleinstreuer, Matthias Roesslein
The use of in vitro assays to inform decision-making requires robust and reproducible results across studies, laboratories, and time. Experiments using positive control materials are an integral component of an assay procedure to demonstrate the extent to

Resources for developing reliable and reproducible in vitro test methods

September 29, 2021
Author(s)
Elijah Petersen, Vytas Reipa, Menghang Xia, Monita Sharma
A broad range of in vitro test methods have been developed given their numerous potential advantages over in vivo tests. We describe here key resources and tools to increase the reliability and reproducibility of in vitro test methods.

Use of cause-and-effect analysis to optimize the reliability of in vitro inhalation toxicity measurements using an air-liquid interface

September 29, 2021
Author(s)
Elijah Petersen, Monita Sharma, Amy Clippinger, John Gordon, Aaron Katz, Peter Laux, Lars Leibrock, Andreas Luch, Joanna Matheson, Andreas Stucki, Jutta Tentschert, Frank Bierkandt
In vitro inhalation toxicology methods are increasingly being used for research and regulatory purposes. While the opportunity for increased human relevance of in vitro inhalation methods compared to in vivo tests has been established and discussed, how to

Quantitative Craniofacial Analysis and Generation of Human Induced Pluripotent Stem Cells for Muenke Syndrome: A Case Report

September 22, 2021
Author(s)
Fahad Kidwai, Byron Mui, Konstantinia Almpani, Priyam Jani, Cyrus Keyvanfar, Kulsum Iqbal, Sriram Paravastu, Deepika Arora, Pamela Orzechowski, Randall Merling, Barbara Mallon, Jeremiah Woodcock, Vamsee Myneni, Moaz Ahmad, Paul Kruszka, Maximilian Muenke, Pamela Robey, Janice Lee
In this case report, we focus on Muenke syndrome (MS), a disease caused by the p.Pro250Arg variant in fibroblast growth factor receptor 3 (FGFR3) and characterized by uni- or bilateral coronal suture synostosis, macrocephaly without craniosynostosis

Towards Absolute Viability Measurements for Bacteria

September 12, 2021
Author(s)
Joy Dunkers, Hariharan K. Iyer, Brynna H. Jones, Charles Camp, Stephan J. Stranick, Nancy Lin
Quantifying viable, vegetative bacteria is a critical measurand in healthcare diagnostics, food safety, and antimicrobial development. Viability determination has traditionally relied on such techniques as plate counting, colorimetric or fluorescent

Critical considerations for the design of multi-organ microphysiological systems (MPS)

September 9, 2021
Author(s)
Mridu Malik, Yang Yang, Parinaz Fathi, Gretchen Mahler, Mandy Esch
The process of identifying new drugs and getting them approved for use in patients requires extensive preclinical studies and clinical trials. Preclinical studies rely on in vitro experiments and animal models of human diseases. The reliability of data

Understanding molt control switches: Transcriptomic and expression analysis of the genes involved in ecdysteroidogenesis and cholesterol uptake pathways in the Y-organ of the blue crab, Callinectes sapidus

September 3, 2021
Author(s)
Tracey Schock, Elena Legrand, J. Sook Chung, Tsvetan Bachvaroff
The crustacean molting process is regulated by an interplay of hormones produced by the eyestalk ganglia and Y-organs (YO). Molt-inhibiting hormone and crustacean hyperglycemic hormone released by the sinus gland of the eyestalk ganglia (EG) inhibit the

The YcnI protein from Bacillus subtilis contains a copper-binding domain

September 1, 2021
Author(s)
Madhura Damle, Stephen Peters, Veronika Szalai, Oriana Fisher
Bacteria require a precise balance of copper ions to ensure that essential cuproproteins are fully metalated while also avoiding copper-induced toxicity. The Gram-positive bacterium Bacillus subtilis maintains appropriate copper homeostasis in part through

Analysis of Seafood Reference Materials: RM 8256, RM 8257, RM 8258 and RM 8259

August 30, 2021
Author(s)
Debra Ellisor, Benjamin Place, Melissa M. Phillips, James H. Yen
NIST Reference Material (RM) 8256 Wild-caught Coho Salmon, RM 8257 Aquacultured Coho Salmon, RM 8258 Wild-caught Shrimp and RM 8259 Aquacultured Shrimp were generated for use in authentication, nutrition and food safety-related determinations of crude fat

Ultraviolet Radiation Technologies and Healthcare Associated Infections: Standards and Metrology Needs

August 20, 2021
Author(s)
Dianne L. Poster, C Cameron Miller, Richard Martinello, Norman Horn, Michael T. Postek, Troy Cowan, Yaw S. Obeng, John J. Kasianowicz
The National Institute of Standards and Technology (NIST) hosted an international workshop on ultraviolet-C (UV-C) disinfection technologies on January 14 – 15, 2020 in Gaithersburg, Maryland in collaboration with the International Ultraviolet Association

Ionization and Structural Properties of mRNA Lipid Nanoparticles that Influence Expression in Intramuscular and Intravascular Administration

August 11, 2021
Author(s)
Manuel Carrasco, Suman Alishetty, Mohamad-Gabriel Alameh, Hooda Said, Lacey Wright, Mikell Paige, Ousamah Soliman, Drew Weissman, Thomas Cleveland, Alexander Grishaev, Michael Buschmann
Lipid Nanoparticles (LNPs) are used to deliver siRNA and COVID-19 mRNA vaccines. The main factor known to determine their delivery efficiency is the pKa of the LNP containing an ionizable lipid. Herein, we report a method that can predict the LNP pKa from
Displaying 101 - 125 of 1166