Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Metrological Framework to Support Accurate, Reliable, and Reproducible Nucleic Acids Measurements

Published

Author(s)

Mojca Milavec, Megan Cleveland, Young-Kyung Bae, Robert Wielgosz, Maxim Vonsky, Jim Huggett

Abstract

Nucleic acid analysis is used in many areas of life sciences such as medicine, food safety, and environmental monitoring. Accurate, reliable measurements of nucleic acids are crucial for maximum impact, yet users are often unaware of the global metrological infrastructure that exists to support these measurements. In this work, we describe international efforts to improve nucleic acid analysis, with a focus on the Nucleic Acid Analysis Working Group (NAWG) of the Consultative Committee for Amount of Substance: Metrology in Chemistry and Biology (CCQM). The NAWG is an international group dedicated to improving the global comparability of nucleic acid measurements; its primary focus is to support the development and maintenance of measurement capabilities and the dissemination of measurement services from its members: the National Metrology Institutes (NMIs) and Designated Institutes (DIs). These NMIs and DIs provide DNA and RNA measurement services developed in response to the needs of their stakeholders. The NAWG members have conducted cutting edge work over the last 20 years, demonstrating the ability to support the reliability, comparability, and traceability of nucleic acid measurement results in a variety of sectors.
Citation
Analytical and Bioanalytical Chemistry

Keywords

nucleic acids, DNA, RNA, measurements, accuracy, traceability

Citation

Milavec, M. , Cleveland, M. , Bae, Y. , Wielgosz, R. , Vonsky, M. and Huggett, J. (2021), Metrological Framework to Support Accurate, Reliable, and Reproducible Nucleic Acids Measurements, Analytical and Bioanalytical Chemistry, [online], https://doi.org/10.1007/s00216-021-03712-x, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932890 (Accessed May 19, 2022)
Created November 4, 2021, Updated January 4, 2022