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Quantitative proteomic analysis for evaluating affinity isolation of extracellular vesicles

Published

Author(s)

Ai Nguyen, Tingting Wang, Illarion Turko

Abstract

Absolute quantification with mass spectrometry and isotope labelled internal standards has found broad applications in biomedical research. In the present research, it was used for developing and evaluating a new affinity-based approach to isolate extracellu-lar vesicles (EVs) from human plasma. First, a phage display peptide library was screened against EVs as a bait and absolute quantification of multiple proteins helped to select the best bait available. Then, absolute quantification was used to evaluate the efficiency of affinity chromatography on peptide-Sepharose. In summary, we have demonstrated that peptides with affinity to EVs selected from phage library screening can be valuable ligands for EVs isolation.
Citation
Journal of Proteomics

Keywords

extracellular vesicles, targeted proteomics, QconCATs, multiple reaction monitoring

Citation

Nguyen, A. , Wang, T. and Turko, I. (2021), Quantitative proteomic analysis for evaluating affinity isolation of extracellular vesicles, Journal of Proteomics, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=932720 (Accessed May 25, 2022)
Created September 28, 2021, Updated January 4, 2022