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Displaying 1 - 25 of 1166

Performing Skin Microbiome Research: A Method to the Madness

March 1, 2027
Heidi H. Kong, Bj?rn Andersson, Thomas Clavel, John E. Common, Scott Jackson, Nathanael David Olson, Julia Segre, Claudia Traidl-Hoffman
Growing interest in microbial contributions to human health and disease has increasingly led investigators to examine the microbiome in both healthy skin and cutaneous disorders, including acne, psoriasis, and atopic dermatitis. The need for common

Comparison of N-glycopeptide to released N-glycan abundances and the influence of glycopeptide mass and charge state on N-linked glycosylation of IgG antibodies

March 7, 2024
Concepcion Remoroza, Meghan Burke Harris, Tytus Mak, Sergey Sheetlin, Yuri Mirokhin, Zachary Goecker, Brian T. Cooper, Mark Lowenthal, Xiaoyu (Sara) Yang, Guanghui Wang, Dmitrii V. Tchekhovskoi, Stephen E. Stein
We report the comparison of mass-spectral-based abundances of tryptic glycopeptides to fluorescence abundances of released labeled glycans and the effects of mass and charge state and in-source fragmentation on glycopeptide abundances. The primary

Digital PCR for the Characterization of Reference Materials

February 14, 2024
Megan Cleveland, Hua-Jun He, Mojca Milavec, Young-Kyung Bae, Peter Vallone, Jim Huggett
Well characterized reference materials are essential to ensuring the harmonization and accuracy of nucleic acid-based tests (such as qPCR); digital PCR (dPCR) can measure the absolute concentration of a specific nucleic acid sequence in a background of non

Establishing a framework for best practices for quality assurance and quality control in untargeted metabolomics

February 12, 2024
Tracey Schock, Jonathan Mosley, Chris Beecher, Warwick Dunn, Julia Kuligowski, Matthew Lewis, Georgios Theodoridis, Candice Ulmer, Dajana Vuckovic, Ian Wilson, Krista Zanetti
Background: Quality assurance (QA) and quality control (QC) practices are key tenets that facilitate study and data quality across all applications of untargeted metabolomics. Such important practices will strengthen this field and accelerate its success

Quantification of mRNA in Lipid Nanoparticles Using Mass Spectrometry

December 26, 2023
Mark Lowenthal, Abigail Antonishek, Karen W. Phinney
Lipid nanoparticle-encapsulated messenger RNA (LNP-mRNA) holds great promise as a novel modality for treating a broad range of diseases. The ability to quantify mRNA accurately in therapeutic products helps to ensure consistency and safety. Here we

Cybersecurity of Genomic Data

December 20, 2023
Ronald Pulivarti, Natalia Martin, Frederick R. Byers, Justin Wagner, Justin Zook, Samantha Maragh, Jennifer McDaniel, Kevin Wilson, Martin Wojtyniak, Brett Kreider, Ann-Marie France, Sallie Edwards, Tommy Morris, Jared Sheldon, Scott Ross, Phillip Whitlow
Genomic data has enabled the rapid growth of the U.S. bioeconomy and is valuable to the individual, industry, and government because it has multiple intrinsic properties that in combination make it different from other types of high value data which

An investigation into an approach for automated supply chain onboarding

November 21, 2023
Elena Jelisic, Nenad Ivezic, Boonserm Kulvatunyou, Perawit Charoenwut, Ana Nikolov
The COVID-19 pandemic has brought the cold chain for bio-pharmaceutical products to the spotlight. To preserve their structure and function, biopharmaceuticals such as cells, proteins and enzymes, RNA molecules, and RNA-based drugs (e.g., mRNA-based COVID

Monitoring Spectrometer Drift with Unsupervised Machine Learning

November 20, 2023
Benjamin Harding, Ziling Hu, Ashley Hiett, Frank Delaglio, Katherine Henzler- Wildman, Chad Rienstra
Solid-state NMR spectroscopy (SSNMR) is a powerful technique to probe structural and dynamic properties of molecules at an atomic level. Modern SSNMR methods employ multidimensional pulse sequences requiring data collection over a period of days to weeks

Metabolomics 2022 Workshop Report: State of QA/QC Best Practices in LC-MS-Based Untargeted Metabolomics, Informed Through mQACC Community Engagement Initiatives

November 8, 2023
Tracey Schock, Warwick Dunn, Julia Kuligowski, Jonathan Mosley, Candice Ulmer Holland, Dajana Vuckovic
Introduction: The Metabolomics Quality Assurance and Quality Control Consortium (mQACC) organized a workshop during the Metabolomics 2022 conference. Objectives: The goal of the workshop was to disseminate recent findings from mQACC community-engagement

Activation loop plasticity and active site coupling in the MAP kinase, ERK2

October 6, 2023
Laurel Pegram, Demian Riccardi, NATALIE AHN
Previous studies of the protein kinase, ERK2, using NMR and hydrogen-exchange measurements have shown changes in dynamics accompanying its activation by phosphorylation. However, knowledge about the conformational motions involved is incomplete. Here, we

Towards Ontologizing a Digital Twin Framework for Manufacturing

September 21, 2023
Milos Drobnjakovic, Guodong Shao, Ana Nikolov, Boonserm Kulvatunyou, Simon P. Frechette, Vijay Srinivasan
This paper presents a timely opportunity to ontologize a digital twin frame-work for manufacturing using recently published international standards and proposes a collaborative research effort to accomplish this task. The ISO/IEC 21838 series of standards

Multi'omic Characterization of Human Whole Stool RGTMs

September 8, 2023
Amanda L. Bayless, Sandra M. Da Silva, Clay Davis, Abraham Kuri Cruz, Tracey Schock, Stephanie Servetas, Paulina Piotrowski
The gut microbiome plays a critical role in a vast and disparate set of health and disease states, including cancer and obesity. Human fecal is a complex mixture including microbes, proteins, undigested plant matter and fat content according to the diet

A multipathway phosphopeptide standard for rapid phosphoproteomics assay development

August 30, 2023
Brian Searle, Allis Chien, Antonius Koller, David Hawke, Anthony Herren, Jenny Kim, Kimberly Lee, Ryan Leib, Alissa Nelson, Jian Min Ren, Paul Stemmer, Yiying Zhu, Ben Neely, Bhavin Patel
Recent advances in methodology have made phosphopeptide analysis a tractable problem for many proteomics researchers. There are now a wide variety of robust and accessible enrichment strategies to generate phosphoproteomes while free or inexpensive

National Institute of Standards and Technology Environmental Scan 2023: Societal and Technology Landscape to Inform Science and Technology Research

August 23, 2023
Ashley Boggs-Russell, Kerrianne Buchanan, David W. Griffith, Heather Evans, Dimitrios Meritis, Lisa Ng, Anna Sberegaeva, Michelle Stephens
The 2023 National Institute of Standards and Technology Environmental Scan provides an analysis of key external factors that could impact NIST and the fulfillment of its mission in coming years. The analyses were conducted through three separate lenses

The complete sequence of a human Y chromosome

August 23, 2023
Arang Rhie, Sergey Nurk, Monika Cechova, Savannah Hoyt, Dylan Taylor, Nathanael David Olson, Justin Zook, Adam Phillippy
The human Y chromosome has been notoriously difficult to sequence and assemble because of its complex repeat structure that includes long palindromes, tandem repeats and segmental duplications1,2,3. As a result, more than half of the Y chromosome is

A Draft Human Pangenome Reference

May 10, 2023
Wen-Wei Liao, Mobin Asri, Jana Ebler, Jennifer McDaniel, Nathanael David Olson, Justin Wagner, Justin Zook, Erik Garrison, Tobias Marschall, Ira Hall, Heng Li, Benedict Paten
Here the Human Pangenome Reference Consortium presents a first draft of the human pangenome reference. The pangenome contains 47 phased, diploid assemblies from a cohort of genetically diverse individuals1. These assemblies cover more than 99% of the
Displaying 1 - 25 of 1166