Skip to main content
U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications

NIST Authors in Bold

Displaying 1 - 25 of 1010

Liver proteome response to torpor in a basoendothermic mammal, Tenrec ecaudatus, provides insights into the evolution of homeothermy

October 6, 2021
Jane Khudyakov, Michael Treat, Mikayla Shanafelt, Jared Deyarmin, Ben Neely, Frank van Breukelen
Many mammals use adaptive heterothermy (e.g., torpor, hibernation) to reduce metabolic demands of maintaining high body temperature (Tb). Torpor is typically characterized by coordinated declines in Tb and metabolic rate (MR) followed by active rewarming

Quantitative Craniofacial Analysis and Generation of Human Induced Pluripotent Stem Cells for Muenke Syndrome: A Case Report

October 5, 2021
Fahad Kidwai, Byron Mui, Konstantinia Almpani, Priyam Jani, Cyrus Keyvanfar, Kulsum Iqbal, Sriram Paravastu, Deepika Arora, Pamela Orzechowski, Randall Merling, Barbara Mallon, Jeremiah Woodcock, Vamsee Myneni, Moaz Ahmad, Paul Kruszka, Maximilian Muenke, Pamela Robey, Janice Lee
In this case report, we focus on Muenke syndrome (MS), a disease caused by the p.Pro250Arg variant in fibroblast growth factor receptor 3 (FGFR3) and characterized by uni- or bilateral coronal suture synostosis, macrocephaly without craniosynostosis

Characteristics to Consider when Selecting a Positive Control Material for an In Vitro Assay

September 29, 2021
Elijah Petersen, Andrew Nguyen, Jeffrey Brown, John Elliott, Amy Clippinger, John Gordon, Nicole Kleinstreuer, Matthias Roesslein
The use of in vitro assays to inform decision-making requires robust and reproducible results across studies, laboratories, and time. Experiments using positive control materials are an integral component of an assay procedure to demonstrate the extent to

Resources for developing reliable and reproducible in vitro test methods

September 29, 2021
Elijah Petersen, Vytas Reipa, Menghang Xia, Monita Sharma
A broad range of in vitro test methods have been developed given their numerous potential advantages over in vivo tests. We describe here key resources and tools to increase the reliability and reproducibility of in vitro test methods.

Use of cause-and-effect analysis to optimize the reliability of in vitro inhalation toxicity measurements using an air-liquid interface

September 29, 2021
Elijah Petersen, Monita Sharma, Amy Clippinger, John Gordon, Aaron Katz, Peter Laux, Lars Leibrock, Andreas Luch, Joanna Matheson, Andreas Stucki, Jutta Tentschert, Frank Bierkandt
In vitro inhalation toxicology methods are increasingly being used for research and regulatory purposes. While the opportunity for increased human relevance of in vitro inhalation methods compared to in vivo tests has been established and discussed, how to

Critical considerations for the design of multi-organ microphysiological systems (MPS)

September 9, 2021
Mridu Malik, Yang Yang, Parinaz Fathi, Gretchen Mahler, Mandy Esch
The process of identifying new drugs and getting them approved for use in patients requires extensive preclinical studies and clinical trials. Preclinical studies rely on in vitro experiments and animal models of human diseases. The reliability of data

The YcnI protein from Bacillus subtilis contains a copper-binding domain

September 1, 2021
Madhura Damle, Stephen Peters, Veronika Szalai, Oriana Fisher
Bacteria require a precise balance of copper ions to ensure that essential cuproproteins are fully metalated while also avoiding copper-induced toxicity. The Gram-positive bacterium Bacillus subtilis maintains appropriate copper homeostasis in part through

Analysis of Seafood Reference Materials: RM 8256, RM 8257, RM 8258 and RM 8259

August 30, 2021
Debra Ellisor, Benjamin Place, Melissa M. Phillips, James H. Yen
NIST Reference Material (RM) 8256 Wild-caught Coho Salmon, RM 8257 Aquacultured Coho Salmon, RM 8258 Wild-caught Shrimp and RM 8259 Aquacultured Shrimp were generated for use in authentication, nutrition and food safety-related determinations of crude fat

Ultraviolet Radiation Technologies and Healthcare Associated Infections: Standards and Metrology Needs

August 20, 2021
Dianne L. Poster, C Cameron Miller, Richard Martinello, Norman Horn, Michael T. Postek, Troy Cowan, Yaw S. Obeng, John J. Kasianowicz
The National Institute of Standards and Technology (NIST) hosted an international workshop on ultraviolet-C (UV-C) disinfection technologies on January 14 – 15, 2020 in Gaithersburg, Maryland in collaboration with the International Ultraviolet Association

Challenges of Accuracy in Germline Clinical Sequencing Data

July 20, 2021
Justin Zook, Ryan Poplin, Mark DePristo
Physicians are increasingly using clinical sequencing tests to establish diagnoses of patients who might have genetic disorders, which means that accuracy of sequencing and interpretation are important elements in ensuring the benefits of genetic testing

MS_Piano: A Software Tool for Annotating Peaks in CID Tandem Mass Spectra of Peptides and N-Glycopeptides

July 15, 2021
Xiaoyu Yang, Pedatsur Neta, Yuri Mirokhin, Dmitrii Tchekhovskoi, Concepcion Remoroza, Meghan Burke, Yuxue Liang, Sanford Markey, Stephen Stein
Annotating product ion peaks in tandem mass spectra is essential for evaluating spectral quality and validating peptide identification. This task is more complex for glycopeptides and is crucial for the confident determination of glycosylation sites in

Universal Spectrum Identifier for mass spectra

July 2, 2021
Eric Deutsch, Yasset Perez-Riverol, Jeremy Carver, Shin Kawano, Luis Mendoza, Tim Van Den Bossche, Ralf Gabriels, Pierre-Alain Binz, Benjamin Pullman, Zhi Sun, Jim Shofstahl, Wout Bittremieux, Tytus Mak, Joshua Klein, Yunping Zhu, Henry Lam, Juan Antonio Vizcaino, Nuno Bandeira
Mass spectra provide the ultimate evidence to support the findings of mass spectrometry proteomics studies in publications, and it is therefore crucial to be able to trace the conclusions back to the spectra. The Universal Spectrum Identifier (USI)

Comprehensive Analysis of Tryptic Peptides Arising from Disulfide Linkages in NISTmAb and Their Use for Developing a Mass Spectral Library

June 22, 2021
Qian Dong, Xinjian Yan, Yuxue Liang, Sanford Markey, Sergey L. Sheetlin, Concepcion Remoroza, William E. Wallace, Stephen Stein
This work presents methods for identifying and then creating a mass spectral library for disulfide-linked peptides originating from the NISTmAb. Analysis involved both partially- reduced and non-reduced proteins under neutral and weakly basic conditions

The mutation spectrum shapes the spectrum of adaptive substitutions

May 20, 2021
Alejandro Cano, Hana Rozhonova, Arlin Stoltzfus, David McCandlish, Joshua Payne
Evolutionary adaptation can be conceived as a series of substitutions representing mutation-fixation events. The changes that are more likely will appear more frequently in the aggregate spectrum of adaptive substitutions. Recent studies establish that

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

May 3, 2021
Peter Vallone, James Huggett, Vladimir Benes, Jeremy Garson, Kathryn Harris, Mikael Kubista, Timothy McHugh, Jacob Moran-Gilad, Tania Nolan, Michael Pfaffl, Marc Salit, Greg Shipley, Jo Vandesompele
Reverse transcription (RT)-PCR, the principal diagnostic method applied in the world-wide struggle against COVID-19, is capable of detecting a single molecule of a viral genome. Correctly designed and practiced RT-PCR assays for SARS-CoV-2 should not cross

Revision of the SNPforID 34-plex forensic ancestry test: Assay enhancements, standard reference sample genotypes and extended population studies

May 3, 2021
John Butler, Manuel Fondevila, Carla Santos, Ana Freire, Christopher Phillips, M Lareu, Peter Vallone
A revision of an established 34 SNP forensic ancestry test has been made by swapping the under- performing rs727811 component SNP with the highly informative rs3827760 that shows a near-fixed East Asian specific allele. We collated SNP variability data for

The San Francisco Estuary Institute Collection at the NIST Biorepository

April 28, 2021
Debra Ellisor, Rebecca Pugh, Nina Buzby, Michael Weaver, Melissa Foley
The National Institute of Standards and Technology (NIST) has been collaborating with the San Francisco Bay Estuary Institute (SFEI) since 2009, providing biobanking services at the NIST Biorepository in Charleston, South Carolina in support of their
Displaying 1 - 25 of 1010