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Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report
Published
Author(s)
Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
Abstract
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and biomanufacturing. Discussion topics highlighted the needs of both start-ups and established industry stakeholders working to bring a tool or product to market. Working groups were convened to address challenges in quality control for cell-free lysates, reagent characterization, analytical measurements and standards needs, and the role of automation for optimization and scale-up. This workshop report summarizes the information gathered at this workshop and seeks to inform future efforts in cell-free manufacturing, as well as the broader establishment of metrics to enable the rapid growth and success of cell-free expression systems for the US and global bioeconomies.
Romantseva, E.
, Piorino Macruz de Oliveira, F.
, Sundberg, C.
, Sittampalam, G.
and Strychalski, E.
(2024),
Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1500-26, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=958740
(Accessed October 1, 2025)