Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report

Published

November 20, 2024

Author(s)

Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski

Abstract

The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and biomanufacturing. Discussion topics highlighted the needs of both start-ups and established industry stakeholders working to bring a tool or product to market. Working groups were convened to address challenges in quality control for cell-free lysates, reagent characterization, analytical measurements and standards needs, and the role of automation for optimization and scale-up. This workshop report summarizes the information gathered at this workshop and seeks to inform future efforts in cell-free manufacturing, as well as the broader establishment of metrics to enable the rapid growth and success of cell-free expression systems for the US and global bioeconomies.

Citation

Special Publication (NIST SP) - 1500-26

Report Number

1500-26

NIST Pub Series

Special Publication (NIST SP)

Pub Type

NIST Pubs

Download Paper

https://doi.org/10.6028/NIST.SP.1500-26

Local Download

Keywords

Automation, biomanufacturing, characterization, CFE, cell-free expression systems, TX-TL, scale-up.

Citation

Romantseva, E. , Piorino Macruz de Oliveira, F. , Sundberg, C. , Sittampalam, G. and Strychalski, E. (2024), Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1500-26, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=958740 (Accessed October 21, 2025)